FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147770
Sequences flagged as poor quality0
Sequence length151
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA69604.710022332002437No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA67174.545577586790282No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT65564.436624483995398No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATCTCCATAACAA57853.9148676998037493No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA55363.7463625905122826No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT50453.414089463355214No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC49883.375516004601746No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC49533.351830547472423No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT44553.01482032888949No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA41192.7874399404479937No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC36352.4599039047167897No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA35462.399675170873655No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG35362.392907897408134No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC34572.3394464370305204No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA33232.2487649725925425No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA31292.1174798673614403No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT30382.0558976788252012No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA29702.0098802192596605No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT28141.9043107531975367No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT26211.7737023753129866No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT23231.5720376260404683No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA23151.5666238072680516No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC22871.5476754415645937No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA13000.8797455505176963No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA12810.866887730933207No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC11750.7951546321986871No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT11630.7870339040400622No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA8990.6083778845503146No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA7920.5359680584692428No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA6460.43716586587263995No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG5800.392501861000203No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA5150.3485145834743182No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT4400.2977600324829126No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA3910.264600392501861No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT3440.23279420721391353No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA3410.23076402517425731No Hit
AATCTAAAACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2920.19760438519320564No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2820.19083711172768492No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATGC2780.18813020234147662No Hit
TAATACCAACTATACCACTCAACTCCAAAAATTACCTTCCAAAAACTCTA2660.18000947418285174No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA2590.1752723827569872No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2530.17121201867767477No Hit
CAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA2460.16647492725181023No Hit
CCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT2310.15632401705352914No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT2270.15361710766732084No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA2160.14617310685524804No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT2110.14278947012248763No Hit
CTCCCAACCTAACCTAAAACCTACTACTCCAACCATCTCCATAACAACCA2060.13940583338972728No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAT1910.12925492319144616No Hit
CAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC1880.12722474115178994No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCAA1830.12384110441902958No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC1800.12181092237937335No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCGTCTCCGA1770.11978074033971713No Hit
CACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA1740.11775055830006091No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA1610.10895310279488393No Hit
CACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC1610.10895310279488393No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAATAAAAAAAAAAAAA1580.10692292075522772No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)