FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229048
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT77223.371345744123502No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA66922.9216583423561873No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC64112.7989766337186963No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA58562.556669344416891No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT56262.456253711012539No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA52832.3065034403269182No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA52012.270703084069715No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT49052.141472529775418No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT46082.011805385770668No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA44261.932346058468094No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA43041.8790821137927423No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA40911.7860885054661033No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC40651.7747371729943069No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC39721.7341343299221126No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC35821.5638643428451677No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC33291.4534071461003808No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT33061.4433655827599454No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA31251.3643428451678252No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT30631.3372742831196955No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG30521.3324717963047046No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC29621.2931787223638713No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG29091.270039467709825No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG28971.2648003911843806No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA27851.2159023436135656No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA27671.2080437288253991No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT27601.2049876008522231No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA25461.1115574028151305No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA24671.0770668156892877No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA24261.0591666375606859No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA23571.0290419475393804No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT21920.9570046453145192No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT20610.8998113932450841No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA17420.7605392756103524No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA17290.7548636093744543No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT16850.7356536621144912No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC16180.7064021515140931No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA15790.6893751528063987No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA15570.6797701791764171No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC14520.6339282595787783No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT13740.5998742621633893No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA13490.5889595194020467No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC13190.5758618280884356No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT10920.47675596381544477No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA10570.46147532394956514No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA10510.45885578568684293No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC10430.45536306800321336No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT10340.45143376060913No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA9620.41999930145646325No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA8610.37590374070063914No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCGAAACATATCCTAAACCA7930.3462156403897873No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCGAAA7520.32831546226118546No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC7520.32831546226118546No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC7210.31478118123712057No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT6720.2933882854248891No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA6310.27548810729628725No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA6050.26413677482449094No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT5690.2484195452481576No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA5510.2405609304599909No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCAAAA5490.2396877510390835No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT5440.237504802486815No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT5050.22047780377912052No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA4940.21567531696412978No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCACATAATACAACCTAAATCC4820.21043624043868533No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG4790.20912647130732423No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT4570.19952149767734273No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA4290.18729698578463902No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA4210.18380426810100942No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA3950.17245293562921307No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT3840.16765044881422234No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA3750.16372114142013902No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACGTCCTCTATTAATC3600.15717229576333344No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA3580.15629911634242605No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT3380.14756732213335197No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA3380.14756732213335197No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3360.14669414271244455No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCGAATATCTA3100.13534281024064823No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA3070.13403304110928713No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCGAAA3060.13359645139883344No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA2990.1305403234256575No Hit
AACTAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCG2960.1292305542942964No Hit
AATCTAAAACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2870.12530124690021305No Hit
AAATTACAAAAATAAACTAAAATCCCAACGCATCGAAAATACAATAATAC2850.12442806747930564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)