FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186660
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT78484.204435872709739No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC46282.479374263366549No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA45242.423657987785278No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT45172.419907853851923No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA42812.293474766955963No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA40132.14989821065038No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA40122.1493624772313296No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC38672.071681131468981No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC38662.0711453980499304No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT36981.981142183649416No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC36861.9747133826208079No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA35171.8841744348012428No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA34001.8214936247723135No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA32421.736847744562306No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG31411.682738669238187No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA29351.5723775849137467No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT28591.5316618450658952No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT27621.4796957034179792No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC26741.4325511625415193No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG26131.3998714239794279No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC25401.360762884388728No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT25291.3548698167791706No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT24211.2970106075216972No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA23721.2707596699882138No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA23061.235401264330869No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA22921.2279009964641594No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA20631.1052180435015535No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT20341.0896817743490839No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA17940.9611057537769206No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCAAATATCTA15100.8089574627665275No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA14980.8025286617379193No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC14400.7714561234329798No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT14340.7682417229186757No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG13840.7414550519661416No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCA13510.7237758491374692No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA13130.7034179792135433No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA13120.7028822457944927No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCGAAA13000.6964534447658844No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT12810.6862745098039216No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTA12090.6477017036322726No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA11640.6235936997749919No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC11520.6171648987463838No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT11220.6010928961748634No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC10250.5491267545269474No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA10000.5357334190506804No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA8890.47626700953605483No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA7860.4210864673738348No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA7590.4066216650594664No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA7300.39108539590699665No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT7260.38894246223079393No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA7040.377156327011679No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA6900.36965605914496946No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC6780.3632272581163613No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC6610.3541197899924997No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT5890.3155469838208508No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTGAAACCCCCTCCTTCC5850.313404050144648No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT5740.30751098253509057No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA5690.30483231543983713No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG5070.27161684345869497No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA4770.25554484088717455No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCGAAACATATCCTAAACCA4720.25286617379192117No Hit
CCCTTTAAACTATCCCACCTTTCTAAACCAAAACAATATATTTCTTAATT4500.2410800385728062No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCGAACACTA4340.2325083038679953No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA4180.22393656916318438No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA3510.18804243008678884No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAACCTTACACACCCCCAAAATTA3430.18375656273438337No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCGAAA3060.16393442622950818No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA2950.1580413586199507No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA2940.15750562520090003No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCGATCCCCTTCCTCCCAT2930.15696989178184936No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA2870.15375549126754529No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT2860.1532197578484946No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2810.15054109075324118No Hit
ACCAAACAACCAAACTAAACAACGCTAAGAACGAAAAACCCAAATATCCT2770.14839815707703846No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT2740.1467909568198864No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA2330.12482588663880853No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA2320.12429015321975785No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAACATCCACGTTACTC2310.12375441980070716No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA2290.12268295296260581No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2250.1205400192864031No Hit
CCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC2190.11732561877209902No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)