FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196466
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAGGAGGGGTTGTATAAGGTTTATTTGGAAGTTAGAATTTATTTGTGG71393.6337076135310946No Hit
AAAGAGATTTATTTTGAGTGATTATGGTTCGTGATATAGTTTTAGGAGAT58492.9771054533608865No Hit
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTTC53962.7465312064173952No Hit
GGATTATTAGGGAAGGGTTTTTTTTCGTTAGCGTTATAAGGGTATTTGAA47762.4309549743976056No Hit
AAAGATAATAAATTGGGTATTGTTGAAATGTAATTAATTAAATAGTTTTT47722.4289189987071556No Hit
TTGTGGGGATTTAGGTTGTATTATGCGGATGAGGTTTTTAGGTAGTAGGT46222.352569910315271No Hit
GGTTGGAGTTTTAGGTGTATTTGGGCGTAGGTTTTTTTGAGGAAATTAAG46072.3449350014760824No Hit
TAGAAAGGTGGGATAGTTTAAAGGGGGATTTTTAGGTTATAGGTAAATTT44502.26502295562591No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTCGGAAGGGAG43152.196308776073214No Hit
TGTGTGTGTGAATTTAAAAGTGTGATAATAAATATTATTTTTATTAGTTG40892.081276149562774No Hit
GTTGGGATTTTAGTTTATTTTTGTAATTTAAATATTTTTTAGTAAAGTTT40892.081276149562774No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTCGGAGGTGTTACGTTTTGTTGT40662.0695692893426854No Hit
TGGGGAGGGAAGAGTAGATTTTACGATATTTTTGGGGTCTTTAGATAGTT39071.9886392556472874No Hit
TGGGGAGGGAAGAGTAGATTTTACGATATTTTTGGGGTTTTTAGATAGTC38571.9631895595166593No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGTATTTTTTTAAAGAG36961.8812415379760365No Hit
GTGTTTGGAGTGTTGTTTTTTTAGGGATGGTGTTTGTAGTGATAGGGTTG35111.787077662292712No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTCGGGGTTTCGTTTTCGTTTAG34531.7575560147811835No Hit
GAAGTTGTGGAATTTAGGTAGGAAGTGGTTGATTTTTAGTGTTTAGTTGT32291.643541376115969No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTA31621.6094387833009274No Hit
TTGGTGGGGAGGTTTTGATAGGTTTTGGGTTTGGGTTTAGGACGGTGAGG31601.6084207954557024No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGCG31381.5972229291582258No Hit
GGTTAGGAGAATTTTAGGATTTTGGGTTTTTTTTATAGTAGATTGGAGGA29651.5091669805462524No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTTGTGGTGG29051.4786273451894985No Hit
GTTTATTGTTTGTTGTGTGTGTGGTGTGTTTATATGTGGTTGGGTATTTG28831.467429478892022No Hit
GGAATTGTTTTTGGGAATTTGTAGCGTTGGTTTCGTTAAGTCGGTTGGAA28361.4435067645292317No Hit
TTGAGGTTATTAGAAGGGAGGTTTTAGTTATATAGGGTGGAAGAATTTAA27491.3992242932619385No Hit
TGGGGAGGGAAGAGTAGATTTTACGATATTTTTGGGGTTTTTAGATAGTT27131.3809005120478863No Hit
GAGAGTAGTAGGAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGCGGT26711.3595227672981585No Hit
TAGATGTAATAGGTTTTTTTGGGGGGATTTTTAGGGAGTGGTTGGGTAGA23891.2159864811214154No Hit
AAAATATTAGAGGAAGGAGGGGTTTGGGTTTTGTTGGTTTTGTTTAAAAA22111.1253855628963791No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT21761.107570775604939No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTCGGTTTGTTTTTAATTTTTTAG20831.0602343408019708No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTTGTTGACGGTTTT19831.0093349485407144No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTCGGTTTTTTTTGTTTGTTTGT19570.9961011065527877No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTCGGTAGGGTTGAGCGTTAGGTT17040.867325644131809No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTTAT14350.7304062789490293No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTTGGGTGGGGAGTAGGAGAAGGGTT14180.7217533822646158No Hit
AAAATATTAGAGGAAGGAGGGGTTTGGGTTTCGTTGGTTTTGTTTAAAAA14170.7212443883420032No Hit
GTTTGGAGGAGTTGGTTTTTTAGAGTTGGCGTTGTTGGTGTTGTATTTTG14040.7146274673480398No Hit
GTTTGGAGGAGTTGGTTTTTTAGAGTTGGTGTTGTTGGTGTTGTATTTTG13990.712082497734977No Hit
GGAATTGTTTTTGGGAATTTGTAGCGTTGGTTTCGTTAAGTTGGTTGGAA13980.7115735038123645No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTTAGGGTTTCGTTTTCGTTTAG12860.6545661844797573No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTCGTTGACGGTTTT12710.6469312756405688No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGTG12470.6347154214978673No Hit
GGAATTGTTTTTGGGAATTTGTAGTGTTGGTTTCGTTAAGTCGGTTGGAA12170.6194456038194904No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTTGGTTTGTTTTTAATTTTTTAG11840.6026488043732758No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGCG11100.564983254099946No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTTGGTTTTTTTTGTTTGTTTGT11010.5604023087964329No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTTGGGTGTAGGGGATAA10530.5359706005110298No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTCGTTGATGGTTTT10100.5140838618386896No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTCGGGTGGGGAGTAGGAGAAGGGTT9890.5033949894638258No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTGGGTGTAGGGGATAAG8720.44384270051815583No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTTGGTAGGGTTGAGTGTTAGGTT8460.4306088585302291No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGTG7590.38632638726293606No Hit
GGGAGATTAAGTAGGTGAGTGGTTTTTTTGTTGGTTTTTTTGTTGGTTTT5930.3018333961092504No Hit
GGGAGATTAAGTAGGTGAGTGGTTTTTTTGTTGGTTTTTTTGTTAGTTTT5420.2758747060560097No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTTAT5210.26518583368114584No Hit
GAGAGTAGTAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGCGGTAGG5060.25755092484195735No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTTGGAGGTGTTATGTTTTGTTGT4570.23261022263394174No Hit
ATTATTTTTTTAGGGAGTTGGGTTGTATTTTATTTGTTGTTTTGTTTTTA4180.21275945965205176No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTAG3990.20308857512241302No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTT3960.20156159335457535No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTATTT3960.20156159335457535No Hit
TGGGGAGGGAAGAGTAGATTTTATGATATTTTTGGGGTTTTTAGATAGTT3400.17305793368827177No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTTTGGGTGTAGGGGATA3180.16186006739079536No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTATT3140.1598240917003451No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTGGGTGTAGGGGATAAGA3010.15320717070638176No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTTGTTTTTTTTTA3010.15320717070638176No Hit
GGTTAGGAGAATTTTAGGATTTTGGGTTTTTTTATAGTAGATTGGAGGAG2320.11808659004611485No Hit
GAGAGTAGTAGGAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGTGGT2060.10485274805818819No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)