FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200199
Sequences flagged as poor quality0
Sequence length151
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA66963.3446720513089474No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA66933.343173542325386No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC66803.3366800033966206No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT63883.190825128996648No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA61573.075439937262424No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA59022.9480666736597083No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT56762.8351789968980863No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT53942.694319152443319No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA49232.459053242024186No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC47252.3601516491091363No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG45592.277234152018741No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC44652.230280870533819No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA44192.207303732785878No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT43532.1743365351475283No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC41882.091918541051654No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA38441.9200895109366183No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT37881.8921173432434726No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG37461.8711382174736138No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC35051.7507579957941846No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA31721.584423498618874No Hit
CCAAAACTCAACAACCTAAACCCCAAAAATAACTACTAAACTACCTACCG30551.5259816482599813No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA28271.4120949655093182No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT28201.4085984445476751No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT22531.1253802466545786No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT20981.0479572825039087No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA20171.0074975399477522No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCAAAA19940.9960089710737816No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA18210.9095949530217433No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC17850.8916128452190071No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC16820.8401640367834005No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA15470.7727311325231394No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCGGAA15160.7572465396930055No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATACCCACATTACTC14200.7092942522190421No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA12730.6358673120245356No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA11140.5564463358957837No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT10400.5194831143012701No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTG8820.44056164116703883No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA8730.43606611421635477No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT8180.40859344951772986No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT7860.39260935369307537No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACGACCCGAACACTA7770.3881138267423913No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC7500.37462724589033913No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG6780.33866303028486655No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA6060.30269881467939397No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA4800.23976143736981703No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA4610.23027088047392844No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT4310.21528579063831488No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT4200.20979125769858992No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT4090.20429672475886493No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA4010.20030070080270132No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3810.19031064091229227No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA3800.18981113791777182No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3680.18381710198352638No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA3660.1828180959944855No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA3530.17632455706571962No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA3510.1753255510766787No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT3400.16983101813695375No Hit
TCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA3300.1648359881917492No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT3270.16333747920818786No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA3050.1523484133287379No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAATAAAAAAAAAAAAA2700.1348658085205221No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA2690.13436630552600162No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCGAACACTA2600.12987077857531754No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA2460.12287773665203122No Hit
CCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC2410.12038022167942897No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2140.10689364082737676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)