FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182321
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT75444.137757032925444No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA54362.98155451099983No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC46292.5389285929761245No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT41072.2526203783436904No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA40012.1944811623455336No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA39642.174187285063158No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA39292.15499037412037No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC36942.0260968292187953No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA35581.9515031181268203No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT35511.9476637359382627No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC33971.8631973277899967No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC30931.6964584441726407No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA30741.686037263946556No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT29731.6306404637973684No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA28741.576340629987769No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG27431.5044893347447634No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC25651.406859330521443No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT24971.3695624749754554No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT23341.280159718299044No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC22771.2488961776207896No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA22521.2351840983759415No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT22261.220923535961299No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA22211.2181811201123294No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA21601.1847236467548994No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG21321.1693661180006691No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG21141.1594934209443784No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA19761.0838027435128152No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA18841.0333422918917734No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA17410.9549091986112406No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT16620.9115790281975197No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA15370.843018631973278No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC14170.7772006515980057No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA13470.7388068297124303No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA13420.7360644138634606No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT13280.7283856494863455No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA12910.7080917722039699No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT11950.6554373879037522No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT11550.6334980611119948No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA10000.5484831697939349No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA9860.5408044054168197No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC9650.5292862588511472No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC9320.5111863142479472No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT8260.4530470982497902No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACAAAACCCTAAAAAA8210.45030468240082056No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA7440.4080714783266876No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA6800.37296855545987573No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC6610.36254737523379094No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA6480.35541709402646976No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT6100.3345747335743003No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG5870.32195962066903977No Hit
ACACTCTAAAATTTCCCCTAAAAAACCAATTCAAACCATAACAAAAAATA5790.3175717553106883No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA5600.3071505750846035No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT5490.30111726021687024No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA5440.29837484436790057No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC5140.28192034927408255No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT4870.2671113036896463No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCAAAA4700.25778708980314935No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA4670.25614164029376757No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA4240.2325568639926284No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT4130.2265235491248951No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA4010.21994175108736788No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCGTCTT4010.21994175108736788No Hit
CCCTTTAAACTATCCCACCTTTCTAAACAAAACCAATATACATCTTAAAT4000.21939326791757396No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACGACCCAAACACTA3900.21390843621963462No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTA3800.20842360452169523No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA3450.18922669357890753No Hit
ACCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA3420.18758124406952573No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA3300.18099944603199852No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT3290.18045096286220455No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTAGTC3280.17990247969241063No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCAAATATCTA3190.17496613116426524No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCACATAATACAACCTAAATCC3100.17002978263611981No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCGAACACTA3050.16728736678715014No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC3010.1650934341079744No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA2850.15631770339127143No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACAAAACACCCAAAA2750.1508328716933321No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA2720.14918742218395029No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCA2710.14863893901415634No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA2560.14041169146724733No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT2420.13273292709013224No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA2410.13218444392033832No Hit
ATATAATACCCACCTTCCAAACCACGAACCCACAACCCAATCCTAAAAAC2400.13163596075054437No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCGAATATCTA2340.12834506173178076No Hit
AAAATTTAATCCTCAATCCAATCCTGGACCACCAGCGTCCTCTGTTGGTC2330.1277965785619868No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA2320.1272480953921929No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA2260.12395719637342928No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA2170.11902084784528387No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAACAACTT2030.11134208346816879No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA2010.1102451171285809No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAATAAAAAAAAAAAAA1970.10805118444940516No Hit
CCCTTTAAACTATCCCACCTTTCTAAGCGAAACCAATATACATCTTAAAT1960.10750270127961123No Hit
CCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC1930.10585725177022942No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCGAAA1900.10421180226084761No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)