FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218839
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGAGGGAAGAGTAGATTTTACGATATTTTTGGGGTTTTTAGATAGTT96754.421058403666622No Hit
GGTAGGAGGGGTTGTATAAGGTTTATTTGGAAGTTAGAATTTATTTGTGG79073.6131585320715227No Hit
GGAATTGTTTTTGGGAATTTGTAGCGTTGGTTTCGTTAAGTCGGTTGGAA63172.88659699596507No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTCGGAGGTGTTACGTTTTGTTGT61532.811656057649688No Hit
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTTC57602.632071979857338No Hit
AAAGAGATTTATTTTGAGTGATTATGGTTCGTGATATAGTTTTAGGAGAT55652.5429653763725844No Hit
AAAGATAATAAATTGGGTATTGTTGAAATGTAATTAATTAAATAGTTTTT48842.231777699587368No Hit
TAGAAAGGTGGGATAGTTTAAAGGGGGATTTTTAGGTTATAGGTAAATTT48552.2185259482998916No Hit
TTGTGGGGATTTAGGTTGTATTATGCGGATGAGGTTTTTAGGTAGTAGGT44892.0512797079131233No Hit
TGTGTGTGTGAATTTAAAAGTGTGATAATAAATATTATTTTTATTAGTTG44752.044882310739859No Hit
GTGTTTGGAGTGTTGTTTTTTTAGGGATGGTGTTTGTAGTGATAGGGTTG41641.9027687021052004No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTCGTTGACGGTTTT40241.8387947303725571No Hit
GTTGGGATTTTAGTTTATTTTTGTAATTTAAATATTTTTTAGTAAAGTTT39691.8136620986204472No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGTATTTTTTTAAAGAG39411.8008673042739183No Hit
GGTTGGAGTTTTAGGTGTATTTGGGCGTAGGTTTTTTTGAGGAAATTAAG38341.7519729115925406No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTTAGGGTTTCGTTTTCGTTTAG37201.6998798203245309No Hit
TTGGTGGGGAGGTTTTGATAGGTTTTGGGTTTGGGTTTAGGACGGTGAGG35361.615799743190199No Hit
GTTTATTGTTTGTTGTGTGTGTGGTGTGTTTATATGTGGTTGGGTATTTG34491.5760444893277707No Hit
AAAATATTAGAGGAAGGAGGGGTTTGGGTTTTGTTGGTTTTGTTTAAAAA34151.5605079533355573No Hit
GGATTATTAGGGAAGGGTTTTTTTTCGTTAGCGTTATAAGAGTATTTGAA32111.4672887373822765No Hit
GGTTAGGAGAATTTTAGGATTTTGGGTTTTTTTTATAGTAGATTGGAGGA31461.4375865362206919No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTTGGTTTTTTTTGTTTGTTTGT30541.3955464976535261No Hit
TTGAGGTTATTAGAAGGGAGGTTTTAGTTATATAGGGTGGAAGAATTTAA29891.3658442964919415No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTTGTGGTGG28571.3055259802868777No Hit
GTTTGGAGGAGTTGGTTTTTTAGAGTTGGTGTTGTTGGTGTTGTATTTTG28421.2986716261726658No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT28341.2950159706450861No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTCGGTTTGTTTTTAATTTTTTAG28221.2895324873537166No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTCGGGTGTGGAGTAGGAGAAGGGTT27671.2643998556016067No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTA26441.2081941518650698No Hit
GAGAGTAGTAGGAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGTGGT25971.1867171756405395No Hit
TAGATGTAATAGGTTTTTTTGGGGGGATTTTTAGGGAGTGGTTGGGTAGA24231.1072066679156822No Hit
GGAGGAGGGTTTAGTAGGTGGTGAGGGGAAGGAGGGGGTTTTAGGGGGTG21530.9838282938598696No Hit
GGATTATTAGGGAAGGGTTTTTTTTCGTTAGCGTTATAAGGGTATTTGAA21300.9733182842180782No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTCGGTAGGGTTGAGCGTTAGGTT19200.8773573266191126No Hit
TGTTTGTTTTGGGATTTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTA19160.8755294988553229No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTCGGAAGGGAG17590.8037872591265726No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTTGGAAGGGAG17180.7850520245477268No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGCTTGGTTTTTTTTTATT16490.7535219956223524No Hit
GGTTGGAGTTTTAGGTGTATTTGGGTGTAGGTTTTTTTGAGGAAATTAAG15630.7142236987008714No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTTGTTGACGGTTTT15420.7046276029409748No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTTAT12900.5894744538222163No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTCGTGGTGG9680.442334318837136No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTTGGGTGTAGGGGATAA9630.4400495341323987No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTTGGTAGGGTTGAGTGTTAGGTT9390.4290825675496598No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTACGGAGGAGAGGGGTTT8870.40532080662039216No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTCGTGGTG8680.3966386247423905No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGCG7510.34317466265153834No Hit
GAAGTTGTGGAATTTAGGTAGGAAGTGGTTGATTTTTAGTGTTTAGTTGT7450.34043292100585365No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTTGGTTTGTTTTTAATTTTTTAG7190.3285520405412198No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGCG6870.3139294184309012No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTTGTTTTTTTTTA6780.30981680596237415No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGCG6760.3089028920804792No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTAG6420.2933663560882658No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTGGGTGTAGGGGATAAG6100.2787437339779473No Hit
ATTATTTTTTTAGGGAGTTGGGTTGTATTTTATTTGTTGTTTTGTTTTTA5580.2549819730486796No Hit
GAGAGTAGTAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGTGGTAGG5570.2545250161077322No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTATGGAGGAGAGGGGTTT4980.22756455659183236No Hit
GAAGTTGTGGAATTTAGGTAGGAGTGGGGGTTGGGAGAGACCAAGTCTCT4660.21294193448151383No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGCG4360.19923322625309017No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTAGGGTTTCGTTTTCGTTTAGT3810.17410059450098017No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.17044493897340052No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTT3660.16724624038676836No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTTAT3560.1626766709772938No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTATT3350.15308057521739726No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTTAT3080.140742737811816No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTATTT2820.12886185734718217No Hit
GTTGTGGTTATTGGTTTTGTGTCGTTTTTTTTTTTGGGTGTAGGGGATAA2620.11972271852823309No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGCTTGGTTTTTTTTATTT2240.1023583547722298No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTTGGTTTTTTTGTTTGTTTGTG2210.10098748394938743No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTGGGTGTAGGGGATAAGA2200.10053052700844No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)