FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230308
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT94234.091477499696059No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA63802.7702033798218038No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC55962.429789672959689No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA51942.2552408079615125No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA50222.1805582090070685No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT49692.157545547701339No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT49582.1527693349775086No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA47942.08156034527676No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC46452.0168643729266895No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA43541.8905118363235318No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC41391.7971585876304774No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA41101.784566754085833No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC40681.7663303055039339No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA40491.758080483526408No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA39241.7038053389374228No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT34541.4997307952828387No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA34221.4858363582680583No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC33691.4628236969623287No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA31481.3668652413290028No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG30571.3273529360682217No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC29891.2978272574118137No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT28741.2478941243899473No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA28581.2409469058825573No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA26471.1493304618163502No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG25431.1041735415183145No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT24651.0703058512947878No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG24391.059016621220279No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT22430.9739131945047502No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT21480.9326640846171214No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA20460.8883755666325096No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT20130.8740469284610175No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA19600.8510342671552876No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC19080.8284558070062698No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC16940.7355367594699272No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA16020.6955902530524342No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA14270.6196050506278549No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA13620.5913819754415826No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA13610.5909477742848707No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT12320.534935825069038No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC12250.5318964169720548No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT11980.520172985740834No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT11500.4993313302186637No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA11030.4789238758532053No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC10840.4706740538756795No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA10350.44939819719679736No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCGAATATCTA10140.4402799729058478No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT8400.36472897163798046No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC8000.3473609253695052No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCATAAAATTC7910.34345311495909825No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA7630.3312954825711656No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT7420.32217725828021604No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA7400.3213088559667923No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA7120.3091512235788596No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCAAATATCTA6640.28830956805668934No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCGAAA6420.2787571426090279No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT6080.2639943032808239No Hit
CCAAAACTCAACAACCTAAACCCCAAAAATAACTACTAAACTACCTACCG5930.25748128593014574No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA5920.25704708477343385No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC5630.24445525122878928No Hit
ACCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA5500.2388106361915348No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA5360.23273181999756845No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCA5110.22187679107977146No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT5070.2201399864529239No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA5060.21970578529621204No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACGACCCAAACACTA4900.2127585667888219No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG4700.2040745436545843No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCAAAA4600.1997325320874655No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCAAAA4140.17975927887871895No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCA4110.1784566754085833No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA4070.17671987078173576No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA4050.17585146846831198No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCAAA3970.17237785921461696No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCGAACACTA3790.1645622383938031No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3690.16022022682668427No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA3600.15631241641627733No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT3580.15544401410285358No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT3560.1545756117894298No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA3510.15240460600587039No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACGAAACCCTAAAAAA3480.15110200253573475No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAACAACTT3380.14675999096861594No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT3140.13633916320753078No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3070.13329975511054762No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA3000.13026034701356445No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA2800.1215763238793268No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2720.11810271462563177No Hit
CTCCCAACCCCCACTCCTAAACACACCGTTCCAAAACATATCCTAAACCA2580.11202389843166542No Hit
AATCTAAAAACCTCCTCTCCAAAACCAAATCTTACACACCCCCAAAATTA2510.10898449033468224No Hit
CCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC2400.10420827761085155No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA2380.1033398752974278No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)