FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203913
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGAGGGAAGAGTAGATTTTACGATATTTTTGGGGTTTTTAGATAGTT95684.692197162515386No Hit
GGTAGGAGGGGTTGTATAAGGTTTATTTGGAAGTTAGAATTTATTTGTGG67813.3254378092617927No Hit
GGAATTGTTTTTGGGAATTTGTAGCGTTGGTTTCGTTAAGTCGGTTGGAA59652.9252671482445947No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTCGGAGGTGTTACGTTTTGTTGT49882.4461412465119925No Hit
GGTTGGAGTTTTAGGTGTATTTGGGCGTAGGTTTTTTTGAGGAAATTAAG49742.4392755734063054No Hit
AAAGAGATTTATTTTGAGTGATTATGGTTCGTGATATAGTTTTAGGAGAT45672.239680648119541No Hit
GGATTATTAGGGAAGGGTTTTTTTTCGTTAGCGTTATAAGGGTATTTGAA44692.1916209363797305No Hit
TTGTGGGGATTTAGGTTGTATTATGCGGATGAGGTTTTTAGGTAGTAGGT44602.1872072893832173No Hit
GTTGGGATTTTAGTTTATTTTTGTAATTTAAATATTTTTTAGTAAAGTTT42512.0847125980197436No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT41292.024883160955898No Hit
TAGAAAGGTGGGATAGTTTAAAGGGGGATTTTTAGGTTATAGGTAAATTT41162.0185078930720453No Hit
TGTGTGTGTGAATTTAAAAGTGTGATAATAAATATTATTTTTATTAGTTG38961.910618744268389No Hit
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTTC38621.8939449667260058No Hit
AAAGATAATAAATTGGGTATTGTTGAAATGTAATTAATTAAATAGTTTTT37591.843433228877021No Hit
GTGTTTGGAGTGTTGTTTTTTTAGGGATGGTGTTTGTAGTGATAGGGTTG36331.7816421709258359No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTCGGAAGGGAG35051.7188703025309813No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGTATTTTTTTAAAGAG34151.674733832565849No Hit
GTTTATTGTTTGTTGTGTGTGTGGTGTGTTTATATGTGGTTGGGTATTTG33881.6614928915763094No Hit
GAAGTTGTGGAATTTAGGTAGGAAGTGGTTGATTTTTAGTGTTTAGTTGT31791.558998200212836No Hit
GGTTAGGAGAATTTTAGGATTTTGGGTTTTTTTTATAGTAGATTGGAGGA31301.5349683443429305No Hit
TTGGTGGGGAGGTTTTGATAGGTTTTGGGTTTGGGTTTAGGACGGTGAGG30361.4888702534904592No Hit
TTGAGGTTATTAGAAGGGAGGTTTTAGTTATATAGGGTGGAAGAATTTAA30281.4849470117157808No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTTAGGGTTTCGTTTTCGTTTAG30151.4785717438319284No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTCGGTTTGTTTTTAATTTTTTAG29981.4702348550607367No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTTGTGGTGG29801.4614075610677104No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTA25451.2480812895695712No Hit
GAGAGTAGTAGGAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGTGGT25241.2377827799110406No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTCGTTGACGGTTTT22911.1235183632235315No Hit
TAGATGTAATAGGTTTTTTTGGGGGGATTTTTAGGGAGTGGTTGGGTAGA22861.1210663371143574No Hit
AAAATATTAGAGGAAGGAGGGGTTTGGGTTTCGTTGGTTTTGTTTAAAAA20861.0229852927473972No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTTGTTGACGGTTTT17300.8484010337742076No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTTGGTTTTTTTTGTTTGTTTGT16780.8228999622387979No Hit
GTTTGGAGGAGTTGGTTTTTTAGAGTTGGTGTTGTTGGTGTTGTATTTTG16610.8145630734676063No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTTGGGTGGGGAGTAGGAGAAGGGTT15670.7684649826151349No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTTGGAAGGGAG15530.7615993095094477No Hit
TGTTTGTTTTGGGATTTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTA13780.6757783956883573No Hit
GTTTGGAGGAGTTGGTTTTTTAGAGTTGGCGTTGTTGGTGTTGTATTTTG13230.6488061084874432No Hit
AAAATATTAGAGGAAGGAGGGGTTTGGGTTTTGTTGGTTTTGTTTAAAAA13090.6419404353817559No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTCGGTTTTTTTTGTTTGTTTGT11450.5615139790008484No Hit
TGGGGAGGGAAGAGTAGATTTTATGATATTTTTGGGGTTTTTAGATAGTT11120.5453306066803No Hit
AAAGATAATAAATTGGGTATTGTTGAAACGTAATTAATTAAATAGTTTTT10620.5208103455885598No Hit
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTTT10300.5051173784898462No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTCGGTAGGGTTGAGCGTTAGGTT9830.48206833306361047No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTACGGAGGAGAGGGGTTT9130.4477399675351743No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGCG9120.44724956231333957No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGTG8910.43695105265480866No Hit
GGAGGAGGGTTTAGTAGGTGGTGAGGGGAAGGAGGGGGTTTTAGGGGGCG8360.40997876545389456No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTTGGGTGTAGGGGATAA8310.4075267393447206No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGCG7940.38938174613683285No Hit
GGTAGGAGGGGTTGTATAAGGTTTATTCGGAAGTTAGAATTTATTCGCGG7810.3830064782529804No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTTAT7710.37810242603463246No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGCG7380.36191905371408395No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTTAT7320.35897662238307515No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTTAGGGTTTCGTTTTTGTTTAG6400.31385934197427334No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTTGTTTTTTTTTA6360.31189772108693414No Hit
TGTTTGTTTTGGGATTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTAT6130.3006184009847337No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGTG5960.29228151221354204No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTCGGTAGGGTTGAGTGTTAGGTT5650.2770789503366632No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTTAGGGTTTTGTTTTCGTTTAG5460.26776125112180194No Hit
ATTATTTTTTTAGGGAGTTGGGTTGTATTTTATTTGTTGTTTTGTTTTTA5390.26432841456895834No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTCGGGTGGGGAGTAGGAGAAGGGTT5350.26236679368161914No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTATTT5330.26138598323794954No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTGGGTGTAGGGGATAAG5220.2559915257977667No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTTGGTTTGTTTTTAATTTTTTAG5020.24618342136107063No Hit
GGAGGAGGGTTTAGTAGGTGGTGAGGGGAAGGAGGGGGTTTTAGGGGGTG4990.24471220569556623No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTCGTGGTG4610.22607680726584375No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTTGGTAGGGTTGAGCGTTAGGTT4570.22411518637850456No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTTGTTGATGGTTTT4530.22215356549116533No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTAG4370.2143070819418085No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTTGGTAGGGTTGAGTGTTAGGTT4330.21234546105446933No Hit
GAGAGTAGTAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGTGGTAGG4210.20646059839245168No Hit
GGATTATTAGGGAAGGGTTTTTTTTTGTTAGCGTTATAAGGGTATTTGAA3450.16918980153300672No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTT3350.16428574931465872No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTATT3270.1603625075399803No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTTGGAGGTGTTACGTTTTGTTGT2950.14466954044126662No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTCGCGGTGG2940.14417913521943182No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTATT2910.14270791955392742No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTCGGAGGTGTTATGTTTTGTTGT2870.1407462986665882No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTTAT2840.1392750830010838No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTAGGGTTTCGTTTTCGTTTAGT2830.138784677779249No Hit
GGTTGGAGTTTTAGGTGTATTTGGGTGTAGGTTTTTTTGAGGAAATTAAG2790.1368230568919098No Hit
AAAGAGATTTATTTTGAGTGATTATGGTTTGTGATATAGTTTTAGGAGAT2790.1368230568919098No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTTGCGGTGG2740.13437103078273577No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTTGGTTTTTTTGTTTGTTTGTG2530.12407252112420493No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGCCGGTAGGGTTGAGCGTTAGGTT2440.11965887412769172No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTTTGGGTGTAGGGGATA2270.11132198535650008No Hit
GGTTAGGAGAATTTTAGGATTTTGGGTTTTTTTATAGTAGATTGGAGGAG2160.10592752791631725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)