FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210689
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGAGGGAAGAGTAGATTTTACGATATTTTTGGGGTTTTTAGATAGTT85464.056215559426453No Hit
GGTAGGAGGGGTTGTATAAGGTTTATTTGGAAGTTAGAATTTATTTGTGG75673.5915496300233993No Hit
GGAATTGTTTTTGGGAATTTGTAGCGTTGGTTTCGTTAAGTCGGTTGGAA59012.8008106735520126No Hit
GGATTATTAGGGAAGGGTTTTTTTTCGTTAGCGTTATAAGGGTATTTGAA54352.579631589689068No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTCGGAGGTGTTACGTTTTGTTGT50282.3864558662293716No Hit
AAAGATAATAAATTGGGTATTGTTGAAATGTAATTAATTAAATAGTTTTT49472.3480105748283013No Hit
GGTTGGAGTTTTAGGTGTATTTGGGCGTAGGTTTTTTTGAGGAAATTAAG49392.3442135090109115No Hit
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTTC48772.3147862489261426No Hit
AAAGAGATTTATTTTGAGTGATTATGGTTCGTGATATAGTTTTAGGAGAT44392.106896895424061No Hit
TAGAAAGGTGGGATAGTTTAAAGGGGGATTTTTAGGTTATAGGTAAATTT42812.0319048455306166No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTCGGAAGGGAG41881.9877639554034618No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTCGTTGACGGTTTT41511.9702025259980351No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGTATTTTTTTAAAGAG40111.9037538741937168No Hit
TGTGTGTGTGAATTTAAAAGTGTGATAATAAATATTATTTTTATTAGTTG38501.8273379246187507No Hit
TTGTGGGGATTTAGGTTGTATTATGCGGATGAGGTTTTTAGGTAGTAGGT36511.7328859124111842No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTCGGTTTGTTTTTAATTTTTTAG36251.7205454485046683No Hit
GAAGTTGTGGAATTTAGGTAGGAAGTGGTTGATTTTTAGTGTTTAGTTGT34651.6446041321568758No Hit
GTGTTTGGAGTGTTGTTTTTTTAGGGATGGTGTTTGTAGTGATAGGGTTG34521.638433900203618No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTCGGGGTTTCGTTTTCGTTTAG34321.6289412356601436No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTTGTGGTGG33201.575782314216689No Hit
GGTTAGGAGAATTTTAGGATTTTGGGTTTTTTTTATAGTAGATTGGAGGA31571.4984170981873757No Hit
TTGGTGGGGAGGTTTTGATAGGTTTTGGGTTTGGGTTTAGGACGGTGAGG31431.4917722330069438No Hit
GTTGGGATTTTAGTTTATTTTTGTAATTTAAATATTTTTTAGTAAAGTTT28641.359349562625481No Hit
TTGAGGTTATTAGAAGGGAGGTTTTAGTTATATAGGGTGGAAGAATTTAA28251.340838866765707No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTA26991.2810350801418204No Hit
TAGATGTAATAGGTTTTTTTGGGGGGATTTTTAGGGAGTGGTTGGGTAGA26531.25920195169183No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT26351.2506585536027035No Hit
GTTTATTGTTTGTTGTGTGTGTGGTGTGTTTATATGTGGTTGGGTATTTG26181.2425897887407504No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTTGGTTTTTTTTGTTTGTTTGT24131.1452899771701417No Hit
TGGGGAGGGAAGAGTAGATTTTATGATATTTTTGGGGTTTTTAGATAGTT23861.132474880036452No Hit
GAGAGTAGTAGGAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGTGGT22421.0641276953234389No Hit
AAAATATTAGAGGAAGGAGGGGTTTGGGTTTCGTTGGTTTTGTTTAAAAA19180.9103465297191595No Hit
GTTTGGAGGAGTTGGTTTTTTAGAGTTGGTGTTGTTGGTGTTGTATTTTG18750.8899373009506905No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTTGGGTGGGGAGTAGGAGAAGGGTT15010.712424473987726No Hit
TGTTTGTTTTGGGATTTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTA14820.7034064426714256No Hit
AAAATATTAGAGGAAGGAGGGGTTTGGGTTTTGTTGGTTTTGTTTAAAAA13870.658316286089924No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTTAT13460.6388563237758023No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGCG12770.6061066311008169No Hit
GTTTGGAGGAGTTGGTTTTTTAGAGTTGGCGTTGTTGGTGTTGTATTTTG9880.4689376284476171No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTACGGAGGAGAGGGGTTT9710.4608688635856642No Hit
GGAGGAGGGTTTAGTAGGTGGTGAGGGGAAGGAGGGGGTTTTAGGGGGTG9480.44995229936066905No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTTGGGTGTAGGGGATAA8760.4157787070041626No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTTGTTTTTTTTTA8170.3877753466009141No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGGGTTTGGTTTTTTTTTATT7940.376858782375919No Hit
GTTGGGATTTTAGTTTATTTTTGTAATTTAAATATTTTTTAGTAAAGTTC7880.3740109830128768No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGCG7720.3664168513780976No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTCGGTTTTTTTTGTTTGTTTGT7560.3588227197433183No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTTGGTAGGGTTGAGCGTTAGGTT7450.35360175424440765No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTCGGTAGGGTTGAGCGTTAGGTT7380.35027932165419173No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTCGGGTGGGGAGTAGGAGAAGGGTT7150.3393627574291966No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTCGGTAGGGTTGAGTGTTAGGTT7020.3331925254759385No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTTAT6750.3203774283422485No Hit
GGAGGAGGGTTTAGTAGGTGGTGAGGGGAAGGAGGGGGTTTTAGGGGGCG6360.3018667324824742No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTCCCGAGTCTGGTTTTTTTTTATT5820.2762365382150943No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTGGGTGTAGGGGATAAG5450.2586751088096673No Hit
ATTATTTTTTTAGGGAGTTGGGTTGTATTTTATTTGTTGTTTTGTTTTTA5040.23921514649554557No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGCG4940.23446881422380855No Hit
GAGAGTAGTAGGAGGAGGAGGAGGAGGGGGTAAGCGAGAGGGGGCGCGGT4610.21880591772707642No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTCGTGGCG4550.2159581183640342No Hit
AAAGAGATTTATTTTGAGTGATTATGGTTTGTGATATAGTTTTAGGAGAT4540.2154834851368605No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTATT4460.21168641931947088No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTTGGTAGGGTTGAGTGTTAGGTT4380.2078893535020813No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGCG4350.20646545382056017No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTCGGGGTTTTGTTTTCGTTTAG4270.20266838800317055No Hit
TTGTGGGGATTTAGGTTGTATTATGTGGATGAGGTTTTTAGGTAGTAGGT3840.18225915923470137No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTT3840.18225915923470137No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTTGGTTTGTTTTTAATTTTTTAG3430.16279919692057962No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTATTT3360.1594767643303637No Hit
TTGAGGTTATTAGAAGGGAGGTTTTAGTTATATAGGGTGGAAGAATTTGA3290.1561543317401478No Hit
GGGGATTGGGATAAAGGAGTATTATTTTTGGTTTTTGTTTGAGTGATTTC3280.1556796985129741No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTTGGAAGGGAG3270.1552050652858004No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTAG3190.1514079994684108No Hit
TTGGTGGGGAGGTTTTGATAGGTTTTGGGTTTGGGTTTAGGATGGTGAGG3160.14998409978688967No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTTGGGGTTTCGTTTTTGTTTAG3140.14903483333254228No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTTGGGCGTAGGGGATAA3120.14808556687819488No Hit
GAGAGTAGTAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGTGGTAGG2920.13859290233472085No Hit
GGTTGGAGTTTTAGGTGTATTTGGGTGTAGGTTTTTTTGAGGAAATTAAG2850.13527046974450493No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGTG2790.13242267038146271No Hit
TGTTTGTTTTGGGATTTTTTTTTATTGTTTAGTTTTATTGGGGTTTAGTA2790.13242267038146271No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTCGTTGATGGTTTT2710.12862560456407313No Hit
GTGTTTGGAGTGTTGTTTTTTTAGGGACGGTGTTTGTAGCGATAGGGTTG2710.12862560456407313No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTCTCGAGTTTGGTTTTTTTTTATT2700.12815097133689943No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGTG2690.1276763381097257No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTTGTTGACGGTTTT2570.12198073938364129No Hit
AAAGATAATAAATTGGGTATTGTTGAAACGTAATTAATTAAATAGTTTTT2470.11723440711190428No Hit
GGTTAGGAGAATTTTAGGATTTTGGGTTTTTTTATAGTAGATTGGAGGAG2440.11581050743038318No Hit
GAGAGTAGTAGGAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGCGTGGT2440.11581050743038318No Hit
AAAGAGATTTATTTTGAGTGATTATGGTTCGTGATATAGTTTTGGGAGAT2440.11581050743038318No Hit
GGTAGGAGGGGTTGTATAAGGTTTATTCGGAAGTTAGAATTTATTTGTGG2370.11248807484016726No Hit
GGAATTGTTTTTGGGAATTTGTAGCGTTGGTTTCGTTAAGTTGGTTGGAA2340.11106417515864615No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTTGGTTTTTTTGTTTGTTTGTG2310.10964027547712504No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGTG2160.10252077706951954No Hit
TTGTGGGGATTTAGGTTGTATTATGCGGGTGAGGTTTTTAGGTAGTAGGT2130.10109687738799843No Hit
GTTGGGATTTTAGTTTATTTTTGTAATTTAAATATTTTTTAGTGAAGTTT2120.10062224416082471No Hit
TGTTTGTTTTGGGATTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTAT2110.10014761093365102No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)