FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210689
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT88774.2133191576209486No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA62362.959812804655203No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC53962.561120893829293No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT50042.3750646687772026No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA48522.3029204182467997No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA42262.0058000180360627No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT40911.941724532367613No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC40471.92084067037197No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA38601.8320842568904878No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA37391.7746536364024699No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC37231.767059504767691No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC36801.7466502759992217No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA34271.626568069524275No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG34091.6180246714351487No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC34081.6175500382079748No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC32981.5653403832188675No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA32201.528318991499319No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT30951.4689898381026063No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG30771.4604464400134796No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATCTCCATAACAA29501.4001680201624196No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA28301.3432120329015753No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA27941.326125236723322No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG27761.3175818386341953No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT26201.243539055195098No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA25381.2046191305668543No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT24751.1747172372549113No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA24471.1614275068940476No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA21611.0256824039223689No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA20510.9734727489332619No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC20410.9687264166615248No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA20370.9668278837528301No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT19210.9117704294006805No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT18710.8880387680419954No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT16700.7926374893800816No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA14840.704355709125773No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA14010.6649611512703558No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT13090.6212948943703752No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC12050.5719330387443103No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC11590.55009991029432No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT11550.5482013773856252No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA10910.5178248508465083No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC10790.5121292521204239No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT10780.5116546188932503No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA10230.48554979139869664No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT10010.47510786040087527No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA9850.46751372876609604No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCGAAA9550.4532747319508849No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCAAAAAA8760.4157787070041626No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT8640.41008310827807815No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC7870.3735363497857031No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC7670.3640436852422291No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA7420.3521778545628865No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT6840.32464912738681184No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA6760.32085206156942225No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA6740.3199027951150748No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT6650.31563109607051154No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT6470.30708769798138486No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA6230.29569650052921603No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA5780.27433800530639946No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA5750.2729141056248784No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG5030.23874051326837187No Hit
CACACACACAAAACTATAATAAATACCCTGAAAAACACTCCGTAAAATTC4980.23636734713250335No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCGAAAATACAATAATAC4420.20978788641077606No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCGATCCCCTTCCTCCCAT4390.208363986729255No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA4220.20029522186730203No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACGACCCAAACACTA4180.19839668895860726No Hit
CACAAAACACCTAAATTATACCCAAAGAAACTAATCCCGCCGAATATCTA4100.19459962314121762No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT4060.19270109023252283No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA4040.1917518237781754No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA3900.1851069585977436No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA3470.16469772982927441No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACGAAACCCCGAAAAA3360.1594767643303637No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA3240.1537811656042793No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCGAA3170.15045873301406337No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA3150.14950946655971598No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3130.14856020010536858No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA3110.14761093365102118No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACACCTACTCCG3080.14618703396950009No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAACATCTA3040.14428850106080526No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2960.14049143524341565No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA2960.14049143524341565No Hit
AAATTACAAAAATAAACTAAAATCCCAACGCATCAAAAATACAATAATAC2930.13906753556189455No Hit
ACCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA2730.1295748710184205No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCA2690.1276763381097257No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT2570.12198073938364129No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCAAA2530.1200822064749465No Hit
AATCTAAAACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2440.11581050743038318No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA2410.11438660774886207No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCAAAA2380.11296270806734096No Hit
CCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC2320.11011490870429874No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2290.10869100902277763No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA2280.10821637579560395No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCAAACACTA2220.10536857643256173No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2140.10157151061517213No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)