FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237482
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT100484.231057511727205No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA69122.9105363774938735No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC64802.728627853900506No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT58632.468818689416461No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA56122.363126468532352No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA53882.268803530372828No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA51202.1559528722176835No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC46471.956779882264761No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG46211.9458316840855305No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA43491.831296687748966No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT41541.749185201404738No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC41261.7373948341347973No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC40101.6885490268736159No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA39841.6776008286943853No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC38151.6064375405293874No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA37671.5862254823523467No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT35631.5003242350999233No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC34451.4506362587480315No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG34221.440951314204866No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT32081.3508392214988925No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT32031.3487337987721175No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA31361.3205211342333314No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA29321.234619886980908No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA28961.2194608433481275No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA28931.2181975897120623No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA28341.1933536015361164No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT27971.1777734733579808No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG25551.075871013382067No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC24941.0501848561154108No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA24911.0489216024793457No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA24591.0354468970279853No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT22820.9609149325001475No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT19870.8366949916204175No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA19020.8009028052652412No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA15800.6653135816609259No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC14620.6156256053090339No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA14220.5987822234948332No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT13910.5857286025888279No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA13460.5667797980478521No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA12460.5246713435123505No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA11660.49098457988394906No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCAAA11610.48887915715717406No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA11580.487615903521109No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT11480.4834050580675588No Hit
CCCTTTAAACTATCCCACCTTTCTAAACAAAACCAATATACATCTTAAAT9510.4004514026326206No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC9500.4000303180872656No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC8280.34865800355395354No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT8190.34486824264575844No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA7980.33602546719330306No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT7260.3057073799277419No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT7140.3006543653834817No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA7010.29518026629386646No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT6270.2640200099375953No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCAAAA6120.25770374175727No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA5460.22991216176383894No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCCACTAACCCATCTCCGA5280.22233263994744865No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACTCAAAA4630.19496214449937257No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT4470.18822479177369233No Hit
ATATAATACCCACCTTCCAAACCACGAACCCACAACCCAATCCTAAAAAC4460.1878037072283373No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA4450.18738262268298228No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA4390.18485611541085217No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA4130.17390791723162177No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA4070.17138140995949167No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCGAATATCTA3880.16338080359774634No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA3630.15285368996387094No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA3590.1511693517824509No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG3540.14906392905567578No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC3530.14864284451032078No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA3460.14569525269283568No Hit
TCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA3450.14527416814748065No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3400.14316874542070557No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA3190.13432596996825025No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3180.13390488542289522No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTGAAACCCCCTCCTTCC3170.1334838008775402No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT3100.1305362090600551No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT3090.13011512451470006No Hit
TAAAACTCACTACTATCCCCCACAAACCAAAACTAAAAAACAAACTCTAA2990.1259042790611499No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA2890.12169343360759974No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCCACTAACCCATCTCCGAA2870.1208512645168897No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA2380.1002181217944939No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)