FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226252
Sequences flagged as poor quality0
Sequence length151
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT96874.281509113731592No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC70423.1124586743984586No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA69363.0656082598164875No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA56342.490143733536057No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA56062.477768152325725No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT53622.369923801778548No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT46552.0574403762176687No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA44321.9588777115782403No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA42741.8890440747485107No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC41731.8444035853826708No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT40821.804182946449092No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA40391.785177589590368No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG38801.7149019677174124No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC38561.704294326679985No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA37781.6698194933083463No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA36221.6008698265650692No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC34711.5341300850379223No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC34321.516892668352103No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC32081.4178880186694482No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA31651.3988826618107244No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC30571.3511482771423016No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG30241.336562770715839No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT29551.3060658027332355No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA29151.2883864010041899No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT28821.2738008945777275No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA23621.0439686721001362No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA23341.0315930908898043No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA22490.9940243622155827No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA19770.8738044304580732No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT19710.8711525201987165No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT19210.8490532680374095No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG18750.8287219560490073No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA18680.8256280607464244No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT17360.7672860350405741No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA14640.6470661032830649No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA13700.605519509219808No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA13670.6041935540901296No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC13520.5975637784417376No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT13310.5882820925339887No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT13150.5812103318423705No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA11990.5299400668281385No Hit
AACTAATCTAAACCTCCTAACCTCAAGCGATCTATCTACTTCTACCTCCG11470.5069568445803794No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA11270.4981171437158567No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT10710.47336598129519303No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA10620.46938811590615775No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT10290.4548026094796952No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC9650.42651556671322244No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA9120.40309035942223714No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA7970.3522620794512314No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCAAAA7650.338118558067995No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA7350.3248590067712108No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA7250.3204391563389495No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA7180.3173452610363665No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCA7160.31646129094991426No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA7140.31557732086346196No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA7040.3111574704312006No Hit
CTCCCAACCCCCACTCCTAAACACACCGTTCCAAAACATATCCTAAACCA6950.30717960504216535No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACGACCCGAACACTA6920.305853649912487No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACGAAACCCTAAAAAA6660.2943620387886074No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC6410.283312412707954No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCAAAAAA6390.2824284426215017No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT6180.27314675671375277No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCACATAATACAACCTAAATCC5810.2567933101143857No Hit
ATATAATACCCACCTTCCAAACCACGAACCCACAACCCAATCCTAAAAAC5610.24795360924986298No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCGAAA5550.24530169899050616No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA5500.2430917737743755No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT5270.23292611778017433No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG5220.23071619256404366No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCGAATATCTA5000.2209925216130686No Hit
AAATTACAAAAATAAACTAAAATCCCAACGCATCGAAAATACAATAATAC4110.1816558527659424No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA4040.17856195746335943No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA3860.17060622668528896No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAACTA3760.1661863762530276No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCAAA3750.16574439120980144No Hit
CCCTTTAAACTATCCCACCTTTCTAAACAAAACCAATATACATCTTAAAT3730.16486042112334917No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA3460.1529268249562435No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT3400.15027491469688667No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA3000.13259551296784117No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2940.12994360270848435No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2890.12773367749235365No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA2630.1162420663684741No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT2590.11447412619556953No Hit
TCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA2360.10430847020136838No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)