FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251455
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC61842.4592869499512835No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA61752.4557077807162315No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA59092.349923445546917No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT57392.2823169155514904No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA55422.203972877850908No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA53762.1379570897377262No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC51122.0329681255095347No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC50622.013083851981468No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT49971.9872342963949814No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA46941.8667355988148973No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC45871.8241832534648346No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA45071.7923684158199278No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT43081.7132290071782228No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA39681.5780159471873696No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC38301.5231353522499056No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT37221.4801853214292817No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT37001.4714362410769324No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA35621.4165556461394682No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC35601.4157602751983456No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC35481.4109880495516096No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA35281.4030343401403829No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA33361.326678729792607No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG32161.278956473325247No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT31871.2674235946789685No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA31741.2622536835616711No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG31521.2535046032093218No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT30641.2185082817999244No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA30151.1990216937424192No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA30051.1950448390368058No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT27281.0848859636913166No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA24510.9747270883458272No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA23110.9190511224672406No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT22570.8975761070569287No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA19800.7874172317114394No Hit
AACTAATCTAAACCTCCTAACCTCAAACGACCTATCTACTTCTACCTCCG19160.7619653615955141No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT19080.7587838778310234No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA18800.7476486846553061No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA18460.7341273786562208No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA17820.7086755085402955No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG17370.6907796623650355No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA17150.6820305820126862No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT15990.6358990674275715No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTA15790.6279453580163449No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC15490.6160147938995049No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT13990.5563619733153049No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACGAAACCCTAAAAAA12440.49472072537829825No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT12410.4935276689666143No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA12230.4863693304965103No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCATAAAATTC12170.48398321767314234No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT12140.4827901612614583No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC11880.4724503390268637No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA11530.458531347557217No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA11480.4565429202044103No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA10500.4175697440893997No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC9290.3694498021514784No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA8820.3507585850350957No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA8330.3312719969775904No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA7990.3177506909785051No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG7400.29428724821538643No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC6640.26406315245272516No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCAAAA6130.24378119345409718No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA4970.19764967886898252No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT4940.19645662245729853No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA4920.19566125151617586No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT4610.18333300192877452No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCGCACAAAAACAAA4400.17498160704698654No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT4270.1698116959296892No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT4250.16901632498856656No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA4230.16822095404744386No Hit
ATATAATACCCACCTTCCAAACCACGAACCCACAACCCAATCCTAAAAAC4140.1646417848123919No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCA4100.16305104293014655No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA4020.15986955916565587No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT3990.15867650275397188No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA3980.15827881728341053No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCAAATATCTA3960.1574834463422879No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA3950.15708576087172654No Hit
ATATCTCCTTACATCCCAACCTCATCTACACAACAAAAAACCAAAAACTT3580.14237139846095723No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA3440.13680380187309857No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT3250.12924777793243325No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTCAAATCCTCC3120.1240778668151359No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3070.12208943946232925No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCGAAA3010.11970332663896126No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATCAAACAAAACGTCTAAAA2930.11652184287447058No Hit
CCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC2620.10419359328706924No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCGATCCCCTTCCTCCCAT2590.10300053687538527No Hit
CAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA2570.1022051659342626No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)