FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000888573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266644
Sequences flagged as poor quality0
Sequence length151
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT95943.5980558347459537No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT84803.180270322977453No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC80053.0021301810653904No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA63772.391578284154153No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA63102.3664511483476094No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA60012.2505662981353414No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT54712.051799402949251No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA53592.009795832645775No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA52561.9711675492416856No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC45681.7131456173774773No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC45411.703019756679318No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT44961.6861433221823856No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG43421.6283884130151063No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG43161.6186375841946563No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC41151.543256176775026No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA40501.5188791047239014No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC40011.5005025427161307No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA39761.4911267457733908No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC37461.4048694139001816No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA37421.4033692863893432No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC36801.3801173099713475No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA36111.3542401104093846No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA33901.271358065435562No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA32171.2064775505918004No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG32061.2023521999369946No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT31111.166724171554582No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA30721.1520979283239074No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA27321.0245870899026417No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT26951.0107109104273864No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT26901.0088357510388384No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA26791.0047104003840326No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA25700.9638319257136856No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT24460.9173279728776946No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT23490.8809498807398629No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA20420.765815094283014No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA18220.6833080811869009No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA17600.6600561047689054No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT17310.6491801803153268No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA16590.6221778851202352No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA16290.610926928788947No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC15900.5963006855582724No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT14690.5509218283554101No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA14640.5490466689668622No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACAAAAAACCCAAATATCCT13990.5246695969157379No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA13010.48791647290019646No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT11970.44891315761839756No Hit
AAATTACAAAAATAAACTAAAATCCCAACGCATCGAAAATACAATAATAC11870.44516283884130153No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCAAATATCTA11760.44103748818649585No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC11520.43203672312146535No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA10940.41028487421430826No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT8660.32477760609651823No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC8080.3030257571893611No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA8040.30152562967852264No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA7890.29590015151287863No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC7260.27227314321717344No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCACATAATACAACCTAAATCC7090.2658976012961102No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT6800.25502167684253163No Hit
TAAAACTCACTACTATCCCCCACAAAATAAAACTAAAAAACAAACTCTAA6050.2268942860143112No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG6030.22614422225889202No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAGCAAAACGTCTAAAA5540.20776766025112134No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCAAAA5410.20289224584089646No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA4970.18639084322167385No Hit
CCAACCACTCCCTAAAAATCCCCCAACTACCTGTAGAACATGTCGTCAGT4930.18489071571083543No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTGAAACCCCCTCCTTCC4770.17889020566748173No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATGATAC4660.17476485501267608No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT4650.17438982313496648No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACCCCCCACAAAAACAAA4260.15976357990429185No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT4210.15788842051574384No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA4160.1560132611271958No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA4090.15338803798322856No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAGTCTTACACACCCCCAAAATTA3640.13651160348629635No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT3590.1346364440977483No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCGAAA3540.1327612847092003No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCGAACACTA3430.12863593405439464No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA3360.1260107109104274No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCGAAA3300.12376051964416974No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACC3300.12376051964416974No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA3210.1203852327447833No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCGATCCTAAAAAC3210.1203852327447833No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA3170.11888510523394488No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAACAACTT3070.11513478645684883No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA3060.11475975457913923No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA2910.10913427641349514No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)