FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000888575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299730
Sequences flagged as poor quality0
Sequence length151
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT114043.8047576151870017No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA86402.882594334901411No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT81342.7137757314916757No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA76302.5456243952890936No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA67252.2436859840523136No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA65322.179294698562039No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC61162.0405031194741934No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA60582.0211523704667536No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC57901.9317385647082372No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC53881.7976178560704634No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT52421.748907349948287No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA51151.7065358822940646No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA49811.661828979414807No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC49731.6591599105861943No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT48191.6077803356354052No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA47851.5964367931138024No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA45931.5323791412271044No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG43271.4436326026757416No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC43221.4419644346578588No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT43121.4386280986220932No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA40931.3655623394388283No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT37331.2454542421512695No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG35361.1797284222466886No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC35161.1730557501751577No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG35071.1700530477429687No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC34701.1577086044106364No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA33301.110999899909919No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA31311.0446068127981851No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTA30941.0322623694658526No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCATAAAATTC29540.9855536649651353No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA25740.8587728956060454No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT23820.7947152437193474No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA22910.7643545857938812No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA21940.7319921262469555No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT20740.6919560938177693No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC20080.6699362759817169No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT19690.6569245654422313No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT18410.6142194641844327No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA17110.5708470957194809No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT17080.5698461949087512No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA16730.5581690187835718No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA16640.5551663163513829No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA15050.5021185733827112No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA12560.4190438060921496No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCAAAA12540.4183765388849965No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC12360.4123711340206186No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCGAAA11890.3966903546525206No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT11580.3863477129416475No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCAATT11480.38301137690588194No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACGACCCGAACACTA11240.3750041704200447No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTACCCACCTCCTAAAACA11130.3713342007807026No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCAA10890.36332699429486537No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA10800.3603242918626764No Hit
ATATAATACCCACCTTCCAAACCACGAACCCACAACCCAATCCTAAAAAC10760.3589897574483702No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGGAAAACCCAAATATCCT10680.3563206886197578No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA10620.35431888699829844No Hit
CCCTTTAAACTATCCCACCTTTCTAAACAAAACCAATATACATCTTAAAT10500.35031528375537985No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA10350.3453107797017315No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA9650.3219564274513729No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA8550.2852567310579522No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT7750.258566042771828No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT7280.24288526340373No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAGC7270.2425516298001535No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA7190.23988256097154104No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT7080.23621259133219896No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCAAA6900.230207186467821No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA6460.21552730791045271No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG6180.2061855670103093No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA5510.1838321155706803No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT4880.1628131985453575No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC4800.16014412971674508No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAGCA4720.15747506088813265No Hit
ACCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA4580.15280419043806093No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA3770.12577986854836018No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT3740.12477896773763053No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3550.11843992926967603No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCAAAA3450.1151035932339105No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCAATTCGAA3410.11376905881960431No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA3370.11243452440529811No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC3310.1104327227838388No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAATAAAAAAAAAAAAA3220.10743002035164982No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC3210.10709638674807327No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAG3150.10509458512661396No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACGCATCTCCGA3070.10242551629800153No Hit
CAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA3010.10042371467654221No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)