FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000888577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277286
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT91623.3041697020404923No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC78212.8205535079304402No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA73152.638070439906811No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA72862.627611924150516No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT71192.5673852989332313No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA61442.215762786437108No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT57692.0805233585539837No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG54781.9755775625166796No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA53461.92797328390182No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA51791.867746658684535No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC50261.8125689721082205No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC46321.6704774132123512No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC45881.6546093203407313No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC45151.6282827117128165No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA45091.6261188808666867No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC42981.5500241627777818No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG42741.5413688393932619No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA39051.4082932423562675No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT38771.3981953650743275No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA35251.2712506221013684No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA34781.2543006138066832No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA33991.2258101743326386No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT33861.2211218741660237No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT33851.2207612356916686No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG33441.2059750582431135No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT33341.2023686734995636No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA31321.129519701679854No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT25540.9210706635026652No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC25230.9098908707976602No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA24670.8896951162337803No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAATATCTAAAA23080.8323535988113356No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA22500.8114365672987458No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT20970.7562588807224311No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA20850.751931219030171No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA19480.7025237480435362No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA17980.6484279768902865No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC16410.5918077364165519No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA16390.5910864594678419No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC16350.5896439055704219No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA15390.555022612032342No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT15040.5424002654299171No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA14520.5236470647634572No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA14310.5160736568020022No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA13980.5041725871482873No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA13950.5030906717252224No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCAAAA13830.49876301003296236No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT13460.4854193864818274No Hit
CTCCAAACTACAAAAAACTCCTTCAAGTCACCTATCCACCTCCTAAGGCA12260.4421427695592277No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT12080.4356512770208377No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC11860.42771723058502775No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACAAAAAACCCAAATATCCT11810.42591403821325274No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA11780.4248321227901877No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT11360.40968530686727783No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA11320.4082427529698578No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT11150.40211189890582283No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC9860.3555895357140281No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT8590.30978844947094336No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC8570.30906717252223337No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAGAAACTCCCCACAAAAACAAA7820.2820192869456085No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA7750.2794948176251235No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAACTA7720.2784129022020585No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT7700.27769162525334856No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA7310.2636267247535036No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTGAAGGGCAGGCTCTGG7280.2625448093304386No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA6960.2510043781510787No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT6820.2459554395101087No Hit
CCCTTTAAACTATCCCACCTTTCTAAACAAAACCAATATACATCTTAAAT6430.23189053901026377No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCAA6360.2293660696897788No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCAAATATCTA5980.21566180766428886No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT5410.19510541462605396No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT5410.19510541462605396No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCGAAA5320.19185966835685897No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA5190.187171368190244No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAGCCCCCTCCTTCC4720.1702213598955591No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG4720.1702213598955591No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA4670.1684181675237841No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA4440.16012348261361917No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA4160.15002560533167922No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTACCCACCTCCTAAAACA4140.1493043283829692No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCAAA3960.14281283584457924No Hit
CCAAAACTCAACAACCTAAACCCCAAAAATAACTACTAAACTACCTACCG3890.14028836652409427No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3700.13343623551134928No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA3650.1316330431395743No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCAAACACTA3550.1280266583960243No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCAAA3400.12261708128069936No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA3400.12261708128069936No Hit
ACCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA3250.11720750416537438No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT3170.11432239637053439No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT3100.1117979270500494No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA2870.10350324213988446No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)