FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242158
Sequences flagged as poor quality0
Sequence length151
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT85473.5295137885182406No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC73583.0385120458543597No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA60792.5103444858315647No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA60662.5049760899908327No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA55022.272070301208302No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT48421.999520973909596No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC45941.8971084994094765No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA45561.8814162654134905No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC43541.797999653119038No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC42251.7447286482379274No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA42191.7422509270806665No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC41521.7145830408245855No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG40641.6782431305180916No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA38551.5919358435401683No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA38451.5878063082780665No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA35341.4593777616267065No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC34791.4366653176851478No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT33671.3904145227496099No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA33191.370592753491522No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA32871.3573782406527968No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG32581.3454025883927023No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG31601.3049331428241064No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT31081.2834595594611782No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA30661.2661155113603515No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA29651.2244072052131252No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT29601.2223424375820744No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA26211.08235119219683No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA26131.0790475639871489No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT26031.0749180287250473No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT25391.048489003047597No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT24231.0005863940072184No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC23160.9564003667027313No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT22840.9431858538640061No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA20380.8415992864163067No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCAAATATCTA18450.7618992558577458No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA17150.708215297450425No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC16950.6999562269262217No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA16850.6958266916641201No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA15360.6342966162588062No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA14530.600021473583363No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTGAAACCCCCTCCTTCC14310.5909364960067394No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT12890.5322970952848967No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA12780.5277546064965849No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT12540.517843721867541No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT12530.517430768341331No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT12500.5161919077627004No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA12260.5062810231336565No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT12230.505042162555026No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA11590.4786131368775758No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT10780.44516390125455285No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACAAAACCCTAAAAAA10240.4228644108392041No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC9180.3790913370609271No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA9020.37248408064156463No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA8520.3518364043310566No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT7930.32747214628465715No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTA6690.27626590903459725No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACGACCCGAACACTA6680.27585295550838707No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA6380.26346434972208227No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT6380.26346434972208227No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC6000.24777211572609617No Hit
ATATAATACCCACCTTCCAAACCACAAACCCACAACCCAATCCTAAAAAC5800.23951304520189298No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCGAAA5740.23703532404463204No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG5740.23703532404463204No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC5680.23455760288737104No Hit
CCTAAAACAAACTAATACCACCCACCAAACGAAAACGAAACCCTAAAAAA5600.2312539746776898No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT5500.22712443941558816No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT4530.18706794737320262No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCAAA4310.1779829697965791No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCACATAATACAACCTAAATCC4140.17096275985100637No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA4070.16807208516753525No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA3870.15981301464333206No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT3830.1581612005384914No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA3820.15774824701228124No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA3690.15237985117154917No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3430.141643059490085No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC3290.13586171012314274No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCAAAA3220.13297103543967162No Hit
TCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA3110.12842854665135986No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA2750.11356221970779408No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2670.1102585914981128No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCAAA2590.10695496328843151No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA2530.10447724213117056No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)