FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357437
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG133413.732405990426285No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT116323.254279775177164No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC113333.170628670227201No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC103852.9054071067069165No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA101802.8480543424435636No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT99172.7744749424374087No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA89562.505616374354082No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT79682.2292040275628993No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA74932.0963134762209847No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC68361.9125048609964834No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC66821.8694203454035256No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA66251.853473479242496No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT65531.833330069354879No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC61321.715547075428677No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG60461.691486891396246No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC58431.6346936662964384No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA56431.578739749941948No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA48551.3582813195052554No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA47341.3244292001107887No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT46721.3070834860408969No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC43151.2072057453481313No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC42491.1887409529511495No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA40741.1397812761409702No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT37821.0580885582634143No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC36531.021998282214768No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA34620.9685622920962296No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC34150.9554131217529244No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC33800.9456211863908884No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA26310.7360737696433217No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT25750.7204066730640645No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA25140.7033407285759449No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC24300.6798400837070588No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA23350.6532619734386759No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT23310.6521428951115861No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA22420.6272434023338378No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA22170.6202491627895266No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT21060.5891947392127844No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACTAAAAAATAAACCCCTA19770.5531044631641381No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC19500.5455506844562817No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC19200.5371575970031083No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA18510.5178534958608091No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA18340.5130974129706773No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA18080.5058234038445936No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA17410.4870788418658393No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA16890.47253082361367177No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC16380.45826257494327677No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA15210.4255295338758998No Hit
ACTCCCACACACACTAACACAATAAAACAACACTACGAACATCAATACAC15040.4207734509857681No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA14900.4168566768409538No Hit
CTCATTCAAAAAACCCAAAAAACCGAACCCAACTCATTCCACACCAACAT14720.41182082436904965No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCAAAACCCAAAAAA13270.3712542350120441No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC11690.3270506410919966No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAGGGCGACCATCAGACA10680.29879391333297894No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT9380.2624238677025602No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCAGAAACATTAAAACCTTCATC8330.23304806161645267No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC8120.2271729003992312No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCATTCTAACACC7860.21989889127314743No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT7170.20059479013084824No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAAACCCCCACAAAAC6420.1796120714979143No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC6150.1720582927900581No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG6060.16954036655410604No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA5680.15890912244675284No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC5560.15555188746548343No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC5550.15527211788371098No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA5300.14827787833939968No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA5190.14520041293990268No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA5000.1398847908862261No Hit
TACTATCCACACTCTCTTACCTCCCTACAATTCCCAAAAAAATCAAATTA4820.13484893841432197No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC4680.13093216426950766No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACGAACC4600.128694007615328No Hit
AACCACTCACCTACTTAATCTCCCAATTCCAATATATCCAAAACTTACCC4480.12533677263405857No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4160.11638414601734012No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA3820.10687198023707674No Hit
CAACTACCAAACCCTAAAAAACCACACACAAACCAAAACCAAATCTCAAC3730.10435405400112469No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)