FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386611
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT125093.235552014815926No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC119293.0855304168789814No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG114682.9662891123118587No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT109862.8416159912677084No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA102922.66210738959833No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA101792.6328790437933733No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT100142.590200485759588No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC96242.4893238940433666No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA95292.464751390933005No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC82512.1341865596167726No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA77932.015721228832082No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA76551.9800264348401881No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT72551.876563263849192No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT65991.7068836634239586No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA65741.7004172152370212No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA65531.694985398759994No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC64581.6704128956496322No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA60951.576520067975303No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC53491.3835612540770956No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA51151.3230352990473628No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG50601.3088091130361008No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC48771.2614747123077201No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC47271.2226760231860967No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC45351.1730137011104185No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA42291.0938643753023063No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC38230.9888492567464454No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA37840.9787615975748234No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC34100.882023532698242No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA28700.7423482518603972No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA27580.7133785639829182No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA27300.7061361420135485No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT26460.6844088761054393No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCACTCTAACCACCCTCG25950.6712173218040873No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC25690.6644922156896725No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT24410.6313840009725539No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA23170.599310417965345No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA22780.5892227587937229No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC22010.5693060983779561No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACAAACATCAATACAC21500.5561145440766042No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC21260.5499067538171444No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC21080.5452509111225495No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAAACCCCCGCAAAAC20930.5413710422103872No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC19900.5147292756802058No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA19580.506452222000926No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT18610.4813624030356094No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT18160.4697227962991224No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC17570.45446197857795045No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACGCCAACAT17470.45187539930317555No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT16380.4236816852081291No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC13810.3572065978464141No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC13180.3409111484153322No Hit
AACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTAATTTATAC12900.33366872644596246No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA12440.3217704617819979No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAGTTAATTTATAC11020.28504103608019427No Hit
CAACTACCAAACCCTAAAAAACCACAAACACTCAACACCAACCCATAAAA10790.279091903748212No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA10280.26590034944686003No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAGTACC9990.2583992695500128No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC9480.2452077152486608No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT9260.23951724084415602No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA8050.2082196316193797No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC7570.19580405110046015No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA6910.1787326278869458No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG6780.1753700748297384No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT6490.1678689949328912No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC6270.1621785205283864No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA6030.15597073026892666No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT5920.15312549306667425No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA5280.1365713857081149No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTCACCC5030.13010493752117763No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT4980.12881164788379015No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC4890.12648372653649276No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA4690.12131056798694295No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC4470.11562009358243816No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC4400.11380948809009572No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4210.10889498746802341No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAACAAATAACT4180.10811901368559096No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC4130.10682572404820348No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA4020.1039804868459511No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACTAAAAATACTAAA3960.10242853928108615No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)