FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000888587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443900
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG162463.6598332957873394No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT139613.145077720207254No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA131852.970263572876774No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT131182.9551700833521064No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC126352.846361793196666No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA114422.5776075692723586No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC112842.542013967109709No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA112512.5345798603289027No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT105162.3690020274836674No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC92242.077945483216941No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC86801.955395359315161No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA85581.9279116918224826No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC85161.9184501013741833No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA84971.9141698580761435No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC81211.8294660959675604No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC79001.7796801081324622No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT75731.7060148682135616No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC75041.6904708267627844No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA65901.4845685965307502No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA64101.4440189231808964No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC56731.2779905384095518No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA50801.1444018923180896No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA48931.1022752872268529No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT46751.053165127280919No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA46501.0475332282045506No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA46291.0428024329804009No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG44441.0011263798152739No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT43160.9722910565442668No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC38070.857625591349403No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC36670.826086956521739No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA34450.7760756927235865No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA31320.7055643162874522No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA28030.6314485244424419No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT25040.564091011489074No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATGTCG24390.5494480738905159No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC23430.5278215814372607No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT23140.5212885785086732No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC23040.5190358188781257No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA22490.5066456409101149No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC21580.4861455282721334No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA21450.4832169407524217No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC20660.4654201396710971No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA20130.4534805136291958No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA19830.4467222347375535No Hit
AACCCCAAATACAAAAAAATACACCACCAAAACCGAAAAATAAACCCCTA19630.4422167154764587No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT19090.4300518134715026No Hit
CAAAACAAACAACACTAAAACCAAACTAACAAAAAACAAATCCCGCATCG18280.4118044604640685No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA17970.40482090560937145No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA16780.37801306600585716No Hit
AAAACCCCACTCTATCTTACAACCTAAATGTAGAACCATGTCGTCAGTGT14940.33656228880378464No Hit
CAACTACCAAACCCTAAAAAACCACTCTACACTAACCGAAACCCAAAAAA13410.3020950664564091No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT13300.29961703086280694No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC11070.24938049110159946No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC9510.21423744086505972No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT9020.20319891867537737No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC8840.19914395134039198No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT8490.19125929263347602No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA8450.19035818878125704No Hit
TAAAACTAAACCCCACATACCTTCCCCAAAAAAACCTACTCATAAACTGT7060.1590448299166479No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCCAAAAATTTCTAAAAT6160.1387699932417211No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA5490.1236765037170534No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC5310.11962153638206804No Hit
TAAAACTAAACCCCACATACCTTCCCCAACCCAAACACAACACTAACACA5250.11826988060373957No Hit
TACTATCCACACTCTCTTACCTCCCGACCCCCACACTCCCTTCTAACACC5190.11691822482541112No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4830.10880829015544043No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA4470.10069835548546971No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)