FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000888588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35803
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTCGCGATGGGTTAG1506242.06910035471888No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGCGTTGAT442712.36488562410971No Hit
TGATTTTGTGAGTTTATAGGTTTAGGTTTTAGTAGTAGAATTATTTTGGT16004.468899254252437No Hit
GGAAATTTGAATTTTTATTTGGTATACACTGACGACATGGTTCTACAGGA7432.0752450911934757No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTT5831.6283551657682318No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAT5001.3965310169538865No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGT4651.2987738457671145No Hit
GGTGGGGAATAAGGTTTTGTATTTATTGAGTTTTTGTTATAGGTTTGGGT3861.0781219450884003No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTT3110.8686422925453176No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGTGTTATTTCGCGATGGGTTAG2840.7932296176298075No Hit
GGAAATATTGTTGTTTTTAAGGTTTGCGCGTTATTTCGCGATGGGTTAGA2280.6368181437309722No Hit
GATAGGTTTGGAGATAGTTTTGTTTTTGTGGTTTTGTATGGTATAGTTTT2270.6340250816970645No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTTGCGATGGGTTAG2180.6088875233918946No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGTGTTTATTTTTTTT2010.5614054688154624No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTG1420.39661480881490374No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGCGTTATTTCGCGATGGGTTAG1410.393821746780996No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGAGTGTAGGTGGCGTTGAT1380.3854425606792727No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGTGTCGGGTA1200.3351674440689328No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGTGTTGAT960.2681339552551462No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGCGTTATTTTGCGATGGGTTAG710.19830740440745187No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTGTTTT670.1871351562718208No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGAGTTATTTCGCGATGGGTTAG660.184342094237913No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTT590.16479066000055861No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATACGTTGTTGGTTGTTTTGA590.16479066000055861No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATCTCGCGATGGGTTAG590.16479066000055861No Hit
GGAAATTTGAATTTTTATTTGGTATACACTGACGATATGGTTCTACAGGA540.15082534983101975No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTCGCGGTGGGTTAG530.14803228779711197No Hit
GGAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTCGCGATGGGTTAGA520.1452392257632042No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTCCGCGATGGGTTAG510.14244616372929642No Hit
TGATTTTGTGAGTTTATAGGTTTAGGTTAGAGATAGTGTTATTGGTTGGA470.13127391559366533No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGCTATTTCGCGATGGGTTAG460.12848085355975755No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTACTTCGCGATGGGTTAG460.12848085355975755No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTCGCGACGGGTTAG430.12010166745803424No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGTGTTATTTTGCGATGGGTTAG410.11451554339021869No Hit
GGAAATATTGTTGTTTTTTAAGGTTGCGCGTTATTTCGCGATGGGTTAGA370.1033432952545876No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTCGTGATGGGTTAG370.1033432952545876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)