FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383842
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGGAAGGCGGGTA145853.799740518234065No Hit
TAGGTTGTTTTGGTTTAGAGAGGGCGGGAGTTTTTGTAGGATAATATAGT107972.812876131324868No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTCGGAAGTATGGTATTT102412.66802486439733No Hit
TGATTTTGTGAGTTTATAGGTTTAGGTTTTAGTAGTAGAATTATTTTGGT101002.631290999942685No Hit
GATTGTTTAGGGAGGGGGTTAATAGCGTGTTAGTATTGAGGTTAGGTTTG94742.4682030627185143No Hit
GTTGGGGATTGAGGTTTTTAGAGTAGAGGAGTTAGTTGTGGGTATTAGTG91482.3832722839084832No Hit
GTGAAGTTTTTGGTTTTTTGTTGTATAGATGAGGTTGGGATGTAAGGAGA86162.2446735896540764No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTATC69851.8197591717425399No Hit
AGAAGGGTTAGTTATAGGGGAGGATGTGGTATAGGTGTTTATGGGATTTT68661.7887568322382648No Hit
TGGTTTTTATTTTTGTGTTTTTTGGGGATGGTTTTTAAGGATAGTATGTT67571.760359731347794No Hit
GGTTGTAGGAGGTTTTTAGTAAGGGAGGGGTTGTAGGAGTGTTTTTGGGG65431.704607625012375No Hit
GTTGGTTTTTTTGGGGTGAAGCGGTTTTTATTTTCGTTGATGAACGGTTT62891.638434564221737No Hit
GTTAAAAGAATTTGGAAGGGTTTGTGAGATATTGGATTGCGGGGTAATAA62311.62332418026167No Hit
GGTGGGGAATAAGGTTTTGTATTTATTGAGTTTTTGTTATAGGTTTGGGT62031.6160295121430173No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTCGGAGGAGAGG58651.5279724469964204No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTCGTGTAGAGGGTTCGTTGGGGTTT58361.520417255016387No Hit
GATAGGTTTGGAGATAGTTTTGTTTAAAGATATTTAGTATTTTAGTGGTA49211.2820379218532625No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTTGGGTA45161.1765257579941746No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGT44941.1707942330438046No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGATTTTTTGT43191.1252025573022233No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTTCGTTATTTTACGATGAGTTAG42681.1119158403718197No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTATGTTGGAGT40351.0512137806701716No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGTGGGGGGTAT38391.0001511038396007No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTATTTTTGATTTTTTTGATT37890.9871249107705775No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGCGTGAAGGTT34630.9021941319605462No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTATGGGATAGGGAGTAT34040.8868232241390989No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTT33540.8737970310700758No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGTGTTTATTTTTTTT31530.8214317349326026No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGCGGGGGGTAT30650.7985056351311217No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTTGTGTAGAGGGTTCGTTGGGGTTT30530.7953793487945562No Hit
GAATTGAAGGAGAATTTATTTGTGGTTGTTATCGTTTTATTAGGATAGTT28870.7521323878053991No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTAGTTTTATTTTTAAATT27870.7260800016673528No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTTGTTTGAGGTTTTTTGT26850.6995065678065454No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTCGGGTA26120.6804883259257716No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTCGGGGCGTAGGTGGTGTTGAT26070.6791857066188692No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGTGTTGAT25410.6619911317677587No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGTGTTATTTTGTGATGGGTTAG24880.648183367114594No Hit
GAATTTTGGGTTTGATTTTTGTTTCGGTTCGGGGCGTAGGTGGTGTTGAT24370.6348966501841904No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGTGTGAAGAAGTGG23730.6182231230558407No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGGTTTTTTGT23260.6059785015709589No Hit
AATTTGTTGTGGGTGATTATGGTGTATGGAGTTAGTGTGGATTTGTGTGG21200.5523105861265833No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTAATTAATTGAGAAGTTGT21060.5486632520672569No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTAATTAATTGAGAAGTTGTT20660.5382422976120382No Hit
GGGGTGTATTTATTAGTTTTAGGGATGTATTTTTAGGTTTCGGGGGTTTA20500.5340739158299509No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTCGTGGCGGGGGGTAT20120.5241740090974932No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGCGTGAAGAAGTGG19470.5072399581077631No Hit
TTTTTAGGTGAGATTAGGGATTTGGGCGTGGGGGGCGGTTAAAGGTATTT17430.45409309038614853No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTAAGGTATTGTGG17370.4525299472178657No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGTGGGGGGTAT16740.43611694395089645No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGCGGGGGGTAT16500.42986437127776533No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTATT15720.40954351009008916No Hit
TTTGGAGAGGAGGTTTTTAGATTTTATTTTGGTGGTATAGTGTTTTTTTT14030.36551497751679074No Hit
TAGGTTGTTTTGGTTTAGAGAGGGTGGGAGTTTTTGTAGGATAATATAGT13940.3631702627643666No Hit
GGGTTTAGGTTGTTGAGTTTTGGTGGGAAGGGTATTTTTTTTCGTTTATC13890.36186764345746425No Hit
GGAGTTGAGTGGTATAGTTGGTATTATTACGTATTAGTAGAGGTTTTTTG12140.3162759677158831No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTAATTAATTGAGAAGTTGTTT11920.3105444427655129No Hit
GGAGTTGAGTGGTATAGTTGGTATTATTATGTATTAGTAGAGGTTTTTTG11730.3055944893992841No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTT11660.30377082236962083No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTCGTGATGGGTTAG11660.30377082236962083No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTG11220.2923077724688804No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTTGTGATGGGTTAG10650.27745791237019396No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGACGTTGAGTGTGAAGAAGTGG10600.2761552930632917No Hit
TTTAAGGTTTGTGTTGATGTTAATGTTATAGGTTACGATAAGGTATGTTA10500.27355005444948705No Hit
GTTGGTTTTTTTGGGGTGAAGCGGTTTTTGTTTTCGTTGATGAACGGTTT9790.2550528602914741No Hit
GAATTGAAGGAGAATTTATTTGTGGTTGTTATTGTTTTATTAGGATAGTT9630.25088447850938667No Hit
GGTTGTAGGAGGTTTTTAGTAAGGGAGGGGTTGTAGGAGTGTTTTTGGAG9600.2501029069252453No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGTTT8760.2282189025692863No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAC7660.1995612778174353No Hit
GTTTATGAGTAGGTTTTTTTGGGGTCGTGTAGAGGGTTCGTTGGGGTTTT7150.18627456088703165No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTATGGGATAGGGAGTATT7100.18497194158012933No Hit
TTTAAGGTTTGTGTTGATGTTAATGTTATAGGTTATGATAAGGTATGTTA7060.18392984613460747No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTTTT7000.18236670296632468No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGCGGGGGGTAT6850.17845884504561774No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTAATTAATTGAGAAGTTG6640.17298784395662797No Hit
GTTTATGAGTAGGTTTTTTTGGGGAATTAATATTTTATTAGAGATTAGAA6460.16829841445177965No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATACGTTGTTGGTTGTTTTGA6380.16621422356073592No Hit
TTTTTTTTGGGTTTTTAGGGTAGAGTTCGGGTTGGGGAGGTTAGTGTTTC6240.16256688950140943No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTGGGGAGTTAAGGTATTGTGG6230.16230636564002898No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAT6220.1620458417786485No Hit
TGGGTTGTGATTAGATGGTAGAAAGGGGTTTTAGTTAGGTTGATAATGGT6130.15970112702622433No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGCGTTATTTTGTGATGGGTTAG6010.15657484068965877No Hit
GGGGATATTTAGGTTTGTTTTTTTTTGGGTTTGGTGTTTTTGGAGTTTTG5920.1542301259372346No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTAGGGTATTGTGG5060.1318250738585147No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTCGAATTTTTTTTTTTCGT5030.1310435022743733No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTAGTTTTATTTTTAAATTT5000.13026193069023193No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTTATTTTTGGGTTTTAGGGA4920.1281777397991882No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTT4900.12765669207642727No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT4490.11697521375982826No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTAATTAATTGAGAAGTTGTTTT4480.1167146898984478No Hit
GTTTATGAGTAGGTTTTTTTGGGGTTGTGTAGAGGGTTCGTTGGGGTTTT4460.11619364217568687No Hit
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGAGTTTAGGAAGGCGGGTA4250.11072264108669713No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTGAGGTATTGTGG4100.10681478316599018No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)