FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383842
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG138503.608255480119424No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT119753.1197732400310545No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC114222.975703544687658No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC108092.8160024176614336No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT103122.686522058555343No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA103042.6844378676642995No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA90602.3603461841070024No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA89712.337159560444141No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC83802.183189958368287No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA81962.135253567874282No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT77862.0284387847082916No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC74441.9393396241161729No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC68791.7921436424362107No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC66491.732223154318704No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA65941.7178943419427786No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC62931.6394766596672588No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT62501.628274133627899No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA61461.6011796520443309No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA54661.4240234263056155No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA53381.3906763720489161No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT51101.3312769316541704No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC47601.2400935801710078No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA46201.203620239577743No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA43431.1314551299753544No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA42841.1160842221539071No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC38120.9931169595823282No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA35830.9334569953262019No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT35020.9123545625543844No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC32900.8571235039417261No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA32290.8412315483975178No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC31350.8167423054277541No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT28860.7518718639440186No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA27390.7135748563210905No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA25410.6619911317677587No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA23970.6244756957289719No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT23840.6210888855310258No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA21700.5653367791956065No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT21130.5504869190969202No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA20780.5413685839486039No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC19910.5187030080085036No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC19630.5114083398898506No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT17490.4556562335544313No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC16650.43377222919847236No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA16000.41683817820874214No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA15930.41501451117907895No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACAAACATCAATACAC15600.40641722375352357No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCAAAAAATAAACCCCTA10380.27042376811292146No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAAACCCCCACAAAAC9610.25036343078662576No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC9540.2485397637569625No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT9460.2464555728659188No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC8270.2154532333616436No Hit
AAACCTATCAAAACCTCCCCACCAAGAACCTACCGCTCTAACCACCCTCG7900.20581385049056644No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC7640.19904023009467436No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC6990.18210617910494423No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT6830.1779377973228568No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT6460.16829841445177965No Hit
TAAAACTAAACCCCACATACCTTCCCCACCAAACATACACAATCCAACTT6310.16439055653107268No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG5660.14745650554134254No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC5380.14016183742268953No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC5290.13781712267026536No Hit
AATACCTTAACTCCCTAAAACCCAAAAATAACTAAATTAACCAAAAATAA5260.13703555108612397No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4950.1289593113833296No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA4830.12583302504676402No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACACCAAAAA4820.12557250118538357No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC4580.11931992851225244No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC3890.10134378207700043No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)