FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339563
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT123793.6455679800213803No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG122533.6084614637048205No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA109153.2144255999623046No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC99882.9414276584904715No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT98332.895780753497878No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT98152.8904798225955126No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA80562.3724610749698876No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC79242.333587581685873No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA77902.2941250960793726No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC77812.2914746306281897No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA72472.1342136805246743No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC71932.1183108878175774No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT69892.0582336709240994No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC66781.96664536477767No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA64161.8894873705321253No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA61881.8223422457688265No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC50811.4963349952733367No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA49831.467474371471568No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT48611.4315458397999783No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA46631.3732355998739556No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT45111.3284721833650899No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC42611.2548481430544554No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG41451.2206865883503208No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACAAACATCAATACAC38821.1432340979435331No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA38771.1417616171373206No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCG36151.0646036228917755No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA34421.0136557869968164No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA32640.9612354702956447No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC30890.9096986420782004No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT29400.8658187140530623No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC29050.8555113484095735No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA25900.7627450576181739No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC25570.7530266842971702No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA25380.747431257233562No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA22350.6581989203770728No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC21400.6302217850590317No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC21100.6213869002217556No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA20900.6154969769969049No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAGGGCGACCATCAGACA20680.609018061449569No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA19180.5648436372631882No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCAAAACCCAAAAAA19130.5633711564569756No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT18640.5489408445560913No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA18320.53951696739633No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA17160.5053554126921955No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA16750.4932810700812515No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA16200.4770837812129119No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC14000.4122946257395535No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC12980.38225601729281455No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA10240.3015640691123591No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA9810.28890073417893No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC9250.2724089491493479No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT8330.24531530231503432No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC8260.24325382918633656No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT6590.19407297025883266No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG5810.17110226968191472No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA5180.1525490115236348No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC5130.1510765307174221No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCATAAATCCCACC5080.1496040499112094No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCAATCCACTC4110.1210379222706832No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC4050.11927094530322797No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA3930.11573699136831753No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAAATCCTATAACTCTAA3810.11220303743340705No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA3660.10778559501476899No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA3540.10425164107985853No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)