FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000888599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414628
Sequences flagged as poor quality0
Sequence length301
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG244255.8908226168999684No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA227995.498663862546668No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT212065.114464049702383No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA168234.057371909277714No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC165053.980676654736294No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT159823.8545394908206876No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC158503.8227037247846263No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA155593.752520331477855No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG151883.663042534512864No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA143903.4705808580221307No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA131583.1734470416855594No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC125493.0265683938373678No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC104512.520572657900576No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC97092.3416170639705953No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC91792.213791639734895No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA91462.2058326982258794No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT89012.1467435870225837No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC71361.7210608063131287No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT50521.2184415910165256No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT47311.1410227963379223No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA38870.9374668377437124No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA18850.4546243861967836No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT17890.4314711018069209No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC16690.40252949631959256No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT16620.4008412359994984No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA14970.36104652845442176No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA10710.25830382897440596No Hit
AAACCTATCAAAACCTCCCCACCACAAACCTACCGCTCTAACCACCCTCG8830.21296198037759148No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC8320.2006617980454769No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG7240.17461435310688134No Hit
AAAACAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACAA7180.17316727283251493No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA6410.1545964093114792No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAAATACCTAAAATA5660.13650790588189896No Hit
AAAAAAATCCTTCCAACCAATAAAACTAACAATACCTACCCAACATGTAG5430.130960764830161No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCGCCCGCAA5060.12203710313823475No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAATACCTAAAATAAA4540.10949574076039245No Hit
CCCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA4310.1039485997086545No Hit
AATACCTAAAAACCCAAAACCCTTCTCCGTTTTAAAAAAAAACAACATTT4240.10226033938856036No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)