FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000888601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178234
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA4084622.91706408429368No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA2843115.951501958100026No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT169009.481917030420684No Hit
CCATAAAAACAAAAACCTAAAAAAACTCCTCGAAAACCTCAACGCACAAA115436.47631764983112No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA101255.680734315562687No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA85234.781915908300324No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA26461.484565234467049No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA19051.0688196415947573No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAATACCTAAAATAAA16370.9184555135383821No Hit
AAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAAA10200.57228138290113No Hit
CAACTACCAAACCCTAAAAAACCACCCAAACAAAACTATCTCCAAACCTA9460.5307629296318324No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC9450.5302018694525175No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC9290.5212249065834802No Hit
TAAAACTAAACCCCACATACCTTCCCCAAAAAAACCTACTCATAAACTGT7280.4084518105411986No Hit
AACCCCAAATACAAAAAATACACCACTAAAACCGAAAAATAAACCCCTAA5930.33270868633369616No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA5790.32485384382328847No Hit
CACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA5070.2844575109126205No Hit
AATACCTAAAAACCCAAAACCCTTCATAAAAAAACCCAAAACCCAACATA4080.22891255316045198No Hit
AACTTACACATCTCAAAAAAACCCAAATAACAAAAAACTATAACCAATCT3770.21151968760169215No Hit
CAACTACCAAACCCTAAAAAACCACACTACCAAACCCTAAAAAACAAAAC3750.21039756724306247No Hit
AATACCTTAACTCCCTAAAACCCAATGTAGAACCATGTCGTCAGTCTAAA3370.18907728042909883No Hit
AAAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATA3320.18627197953252464No Hit
CAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA2890.1621463918219868No Hit
AAAAATCCTAAAAACCCCAAAACCTACCTATGTAGAACTATGTCTCAGTG2870.16102427146335715No Hit
CAAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATA2580.144753526263227No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAGCTCATTCCACACCAACAT2580.144753526263227No Hit
CTAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA2330.13072702178035617No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG2230.12511641998720782No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACCAAA2170.12175005891131882No Hit
AAAAAAAATCCCAAAACAAACATATGAAATCAAAAAAATACCTAAAATAA2130.11950581819405949No Hit
AAAAAAAATCCCAAAACAAACATACGAAGTCAAAAAAATACCTAAAATAA2060.11557839693885567No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAGACCCCTA2020.11333415622159632No Hit
AAAAAAAATCCCAAAACAAACATACGGAATCAAAAAAATACCTAAAATAA1980.11108991550433699No Hit
CTCATTCAAAAAACCCAAAAACCAAACCCAACTCATTCCACACCAACATA1950.1094067349663925No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAGAAATACCTAAAATAA1920.10772355442844798No Hit
CCACACTCCTAAAAACCTAACTTCCCATAATCACCCACAACAAATTTGTA1900.10660143406981833No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)