FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000888603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412962
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG154243.734968350598845No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT142583.4526179164184603No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC141563.4279183072534516No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA127283.082123778943341No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC113752.754490727960442No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC100792.4406603997462235No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC95542.3135300584557417No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA94542.289314755352793No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG87082.1086685942047936No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT83212.0149553711963812No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC79381.9222107603120868No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA78661.9047757420779636No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA76781.8592509722444195No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA76121.8432688721964734No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT76031.8410894949172079No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT73751.7858786038424845No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA73001.7677171265152725No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC72501.755609474963798No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT67671.6386495609765548No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC66691.6149185639356647No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA64471.5611605910471182No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA59901.4504966558666414No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC54711.3248192327623365No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTGTCTACTTCTGCCTCCGCCT47721.1555542640727234No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA46251.1199577685113884No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC43091.0434374107060698No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA40980.9923431211588476No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT40040.9695807362420755No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA39720.961831839249132No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA39060.9458497392011855No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT38980.9439125149529496No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC35100.8499571389135078No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA32890.7964413190559907No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA32010.7751318523253956No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA30490.7383245916089132No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT30220.731786459771117No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT27150.6574454792450637No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAAAGCT26660.6455799807246188No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA25190.6099834851632838No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC25110.6080462609150479No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA24880.6024767412013696No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC24620.5961807623946029No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC22740.5506559925610588No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC19830.4801894605314775No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA19160.46396520745250164No Hit
TAAAACTAAACCCCACATACCTTCCCCAACCCAAACACAACACTAACACA18770.4545212392423516No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT18300.4431400467839656No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC17130.41480814215351536No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA13820.33465548888275437No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA11240.27218000687714605No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGGCCTTAAAAAACAATACC11150.2700006295978807No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC10800.2615252735118485No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC10300.2494176219603741No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT10250.24820685680522664No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAACAAACCACCCCCTAA10130.24530102043287275No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT10100.2445745613397843No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT7820.1893636702650607No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC7040.17047573384476053No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA6790.16442190806902332No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC6450.15618870501402066No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA6400.1549779398588732No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCA6220.1506191853003424No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA5710.13826938071783845No Hit
AAACCTATCAAAACCTCCCCACAAAAACCTACCGCTCTAACCACCCTCGC5470.1324577079731307No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA5130.12422450491812807No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC4680.11332761852180104No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAGCACCTATTAACCTATCCC4520.10945317002532921No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAATAATCACAC4420.10703163971503432No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)