FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384612
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG161604.201636974405375No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT126213.2814888771021185No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT112322.9203456990421515No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA105532.74380414547648No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA104862.726383992179131No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA94622.460141649246513No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC90252.3465206493817146No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC81292.113558599315674No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT76891.9991575925868148No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA75711.9684773226004388No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC73721.9167368672844323No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC73111.900876727715204No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC69761.8137759612284587No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT67421.752935425831747No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC67131.7453953594791634No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA62581.6270943184300022No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA61761.6057741308123514No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA59881.5568937006645658No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG59661.5511736503281228No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC59321.5423335725354381No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA58661.5251734215261095No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC51511.3392717855917133No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC47791.2425509344482233No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA41661.0831695318918806No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA41131.0693894106268136No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA40581.055089284785706No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCCATCCC39721.0327290880159745No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT38951.0127089118384243No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA36810.9570684222021154No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT36410.94666833068131No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC33110.8608675756346655No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA29280.761286699322954No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT28920.7519266169542291No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA28120.7311264339126184No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC25470.6622258275872828No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC25160.6541657566586586No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT24480.6364856010732894No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA22460.5839651388932223No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATGA20600.5356047133214773No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC20580.535084708745437No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT19510.5072644639272826No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA18620.4841242602934906No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA16100.4186036837124167No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA15420.40092352812704757No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA14730.38298337025365825No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC14720.3827233679656381No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC14250.3705032604286918No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC12420.32292284172100716No Hit
AAACTACAAAATCAAAACCAACCCGAAACCGCACCAAACCCCCACAAAAC12240.3182428005366447No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCCATCCCG12050.3133027570642622No Hit
ACTCCCACACACACTAACACAATAAAACAACACTACGAACATCAATACAC11610.3018626563913762No Hit
CTCATTCAAAAAACCCAAAAAACCGAACCCAACTCATTCCACACCAACAT11480.2984826266471145No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA11330.2945825923268125No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC10020.2605222925961749No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC9280.2412821232826849No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCAAAAAATAAACCCCTA9160.23816209582644327No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC8960.23296205006604057No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT8950.23270204777802048No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT8830.22958202032177882No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC8500.2210019448171144No Hit
AAACTACAAAATCAAAACCAACCCAAAACCGCACCAAACCCCCACAAAAC8380.21788191736087278No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT7390.19214169084687946No Hit
ATAATACTCCTTTATCCCAATCCCCAGAACCCTTCACCAAAAATACTAAA6260.16276143230060425No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACCCCTAAAACCTCAACAAA6130.15938140255634248No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA6100.1586013956922821No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCCAACCACCCTCG5820.15132133162771833No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA5670.1474212973074163No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACGCCAAACCCCCACAAAAC5500.14300125841107403No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCGTCAAACA5480.14248125383503374No Hit
AACCACTCACCTACTTAATCTCCCAATTCCAATATATCCAAAACTTACCC5400.14040123553087266No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA5210.1354611920584901No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA5070.13182116002620822No Hit
AACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTAATTTATAC5000.1300011440100673No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAAACCCCCACAAAAC4880.1268811165538257No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA4790.12454109596164446No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCGATCCACTC4710.12246107765748339No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT4690.12194107308144311No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT4630.1203810593533223No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA4570.1188210456252015No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT4470.11622102274500017No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACACACACTTCAAT4270.11102097698459748No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAGATCCCACC4260.11076097469657734No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG4190.10894095868043639No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT3850.10010088088775182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)