FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282388
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG107223.7969035511423996No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT99203.512897148604048No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT81592.8892870801875437No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA78962.796152811025964No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT75142.6608779409889936No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC67592.39351530518294No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA67422.387495219343598No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA64132.270988852217516No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC62972.2299106194314207No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC59382.102780571412383No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA58922.0864909273765173No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA55121.9519243027324107No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA54551.9317393090357946No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT51371.8191282915704634No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC49611.7568026969984563No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC46861.6594189554796948No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA40731.4423417425669647No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG39401.3952434239415272No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC36681.2989220505120616No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC35371.2525319772794878No Hit
AAAAAAAATCCCAAAACAAACATACAAAATCAAAAAAATACCTAAAATAA34811.2327011062793036No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC34781.231638738190008No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA30881.093530886581583No Hit
AAACTACAAAATCAAAACCAACCCGAAACCGCGCCAAACCCCCGCAAAAC28721.017040384152301No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT28501.0092496848308001No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA25260.8945139311868777No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA25260.8945139311868777No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA24600.8711418332223749No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT24170.855914557275805No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA24080.8527274530079182No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC23010.8148363244897092No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACTAAAAAATAAACCCCTA22640.8017337847217304No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC22610.8006714166324348No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT22560.7989008031502756No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA22180.7854441406858648No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA22160.7847358952930011No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA22070.7815487910251143No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT20900.7401164355425868No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGAACATCAAACA19720.6983299573636274No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA19610.6944346077028769No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT18860.6678754054704874No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA18790.6653965465954644No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC18290.6476904117738714No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA17400.6161734917914359No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCGATCCACTC16950.6002379704520022No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC16430.5818235902375455No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC15220.5389747439692905No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC13610.48196098984376107No Hit
CTCATTCAAAAAACCCAAAAAACCGAACCCAACTCATTCCACACCAACAT12050.42671784920039096No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCCAAA11530.4083034689859343No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT11320.4008668923608652No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC11210.39697154270011475No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA10460.37041234046772525No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA10330.3658087454141111No Hit
CAACTACCAAACCCTAAAAAACCACTCTACACTAACCGAAACCCAAAAAA10100.3576639233961783No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC9010.3190645494851056No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC8680.3073785005028542No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT7940.28117342096689657No Hit
AACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTAATTTATAC7570.2680708811989178No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC6800.24080343357366457No Hit
ACTCCCACACACACTAACACAATAAAACGACACTACGAACATCAATACAC5680.2011416915732963No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC5500.19476748303752284No Hit
ATAATACTCCTTTATCCCAATCCCCCAAACCCTTCACCAAAAATACTAAA4980.17635310282306613No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA4730.16750003541226965No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA4710.16679179001940592No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC4680.16572942193011034No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACACTA4520.1600634587872006No Hit
CCTAAAAAAACAACACTCCAAACACGTATACCCAAACCCTAAATCCCGCC4260.15085626867997223No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA4070.14412793744776692No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA3990.14129495587631202No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC3860.13669136082269787No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT3750.1327960111619474No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT3660.12960890689406065No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC3390.12004759409040043No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA3180.11261101746533138No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA2910.10304970466167118No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)