FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402289
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGGAAGGCGGGTA140633.4957455958278745No Hit
TAGGTTGTTTTGGTTTAGAGAGGGCGGGAGTTTTTGTAGGATAATATAGT110672.751007360380224No Hit
GATTGTTTAGGGAGGGGGTTAATAGCGTGTTAGTATTGAGGTTAGGTTTG104982.6095667542488115No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTATC104262.5916691731566113No Hit
TGATTTTGTGAGTTTATAGGTTTAGGTTTTAGTAGTAGAATTATTTTGGT98672.4527143421768924No Hit
GGTTGTAGGAGGTTTTTAGTAAGGGAGGGGTTGTAGGAGTGTTTTTGGGG89052.2135827725838886No Hit
TGGTTTTTATTTTTGTGTTTTTTGGGGATGGTTTTTAAGGATAGTATGTT85602.127823529850431No Hit
GTTGGGGATTGAGGTTTTTAGAGTAGAGGAGTTAGTTGTGGGTATTAGTG85452.124094867122889No Hit
GTGAAGTTTTTGGTTTTTTGTTGTATAGATGAGGTTGGGATGTAAGGAGA85072.1146489215464506No Hit
GTTAAAAGAATTTGGAAGGGTTTGTGAGATATTGGATTGCGGGGTAATAA83422.0736336315434922No Hit
AGAAGGGTTAGTTATAGGGGAGGATGTGGTATAGGTGTTTATGGGATTTT82562.0522559652389205No Hit
GGTGGGGAATAAGGTTTTGTATTTATTGAGTTTTTGTTATAGGTTTGGGT73571.8287847791015912No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTCGGGTA72791.8093957329183747No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTCGGAAGTATGGTATTT72511.8024355624936303No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTCGGAGGAGAGG70601.7549572570962666No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGGTTTTTTGT69841.736065365943389No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTCGTGTAGAGGGTTCGTTGGGGTTT64821.6112794533283286No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTTCGTTATTTTACGATGAGTTAG51791.2873829510625445No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGTGTTGAT51661.2841514433653418No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTATTTTTGATTTTTTTGATT50971.2669995948186503No Hit
GATAGGTTTGGAGATAGTTTTGTTTAAAGATATTTAGTATTTTAGTGGTA47241.174280181660448No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGT46551.1571283331137565No Hit
GTTGGTTTTTTTGGGGTGAAGCGGTTTTTATTTTCGTTGATGAACGGTTT45311.1263047212327455No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTATGGGATAGGGAGTAT43441.07982072589606No Hit
GAATTGAAGGAGAATTTATTTGTGGTTGTTATCGTTTTATTAGGATAGTT41451.0303538003773407No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTT39990.994061483162602No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTAAGGTATTGTGG38540.9580177434630329No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTAGTTTTATTTTTAAATT38460.9560291233416772No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTTGTGTAGAGGGTTCGTTGGGGTTT36600.9097937055201609No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGCGTGAAGGTT34770.8643040202441529No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGCGGGGGGTAT34320.8531180320615278No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTTGTTTGAGGTTTTTTGT31440.7815277076927283No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTATGTTGGAGT29480.7328065147195175No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGTGTTATTTTGTGATGGGTTAG28990.7206262164762149No Hit
TAGGTTGTTTTGGTTTAGAGAGGGTGGGAGTTTTTGTAGGATAATATAGT28120.6989999726564733No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGTGTTTATTTTTTTT27990.6957684649592706No Hit
GGGGTGTATTTATTAGTTTTAGGGATGTATTTTTAGGTTTCGGGGGTTTA26250.6525159773197875No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTACGTTGGAGT20840.5180355416131189No Hit
AATTTGTTGTGGGTGATTATGGTGTATGGAGTTAGTGTGGATTTGTGTGG19960.4961607202782079No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTAATTAATTGAGAAGTTGT19630.48795766227761633No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGCGTGAAGAAGTGG19600.48721192973210803No Hit
TTTGGAGAGGAGGTTTTTAGATTTTATTTTGGTGGTATAGTGTTTTTTTT19440.4832346894893969No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTAATTAATTGAGAAGTTGTT18780.4668285734882137No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGTGTGAAGAAGTGG18280.45439969772974154No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGACGTTGAGCGTGAAGAAGTGG18000.44743952730499714No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGTGGGGGGTAT16430.4084128574233946No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTT16280.404684194695853No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGTGGGGGGTAT16090.39996122190763356No Hit
TTTAAGGTTTGTGTTGATGTTAATGTTATAGGTTACGATAAGGTATGTTA15990.3974754467559391No Hit
TTTTTAGGTGAGATTAGGGATTTGGGCGTGGGGGGCGGTTAAAGGTATTT15720.39076385384636414No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTTGGAAGTATGGTATTT15500.3852951485126364No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGCGGGGGGTAT15370.3820636408154337No Hit
GGAGTTGAGTGGTATAGTTGGTATTATTACGTATTAGTAGAGGTTTTTTG15230.37858355560306145No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTG12040.29928732826400917No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGTTT11930.29655297559714533No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGCGGCGTTGAT11870.29506151050612867No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGCGGGGGGTAT11760.2923271578392648No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAC11740.2918300028089259No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTTTT11640.2893442276572315No Hit
GTTTATGAGTAGGTTTTTTTGGGGTTGTTGTATTGTGTTATGGATGTTTT11630.28909565014206207No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTATT11540.28685845250553704No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTAATTAATTGAGAAGTTGTTT10360.2575263057155428No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTTGGGTA9370.23291713171376796No Hit
GGAGTTGAGTGGTATAGTTGGTATTATTATGTATTAGTAGAGGTTTTTTG9040.22471407371317637No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATACGTTGTTGGTTGTTTTGA8990.2234711861373291No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTTTGTTATTTTACGATGAGTTAG8450.21004800031817922No Hit
GAATTTTGGGTTTGATTTTTGTTTCGGTTCGGGGCGTAGGCGGCGTTGAT7990.19861343462038486No Hit
GTTGGTTTTTTTGGGGTGAAGTGGTTTTTATTTTCGTTGATGAACGGTTT7940.19737054704453763No Hit
TGGGTTGTGATTAGATGGTAGAAAGGGGTTTTAGTTAGGTTGATAATGGT7870.19563050443835153No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGCGTAGGCGGCGTTGAT7640.18991322158945437No Hit
GGGGATATTTAGGTTTGTTTTTTTTTGGGTTTGGTGTTTTTGGAGTTTTG7550.18767602395292937No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTATGGGATAGGGAGTATT7410.18419593874055715No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTTGTGATGGGTTAG7080.17599288073996555No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGCGTTTATTTTTTTT6780.16853555528488226No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTCGTTTATCG6590.16381258249666283No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTCGTGTAGAGGGTCCGTTGGGGTTT6580.16356400498149343No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGCGTTGAT6210.154366636920224No Hit
GTTGGTTTTTTTGGGGTGAAGCGGTTTTTATTTTCGTTGATGAATGGTTT5880.1461635789196324No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTAATTAATTGAGAAGTTG5870.14591500140446295No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTTGGAGGAGAGG5790.14392638128310742No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTT5670.14094345110107412No Hit
TTTTTTTTGGGTTTTTAGGGTAGAGTTCGGGTTGGGGAGGTTAGTGTTTC5480.1362204783128547No Hit
GGGGATATTTAGGTTGTAAGATAGAGTGGGGTTTTAGACCAAGTCTCTGC5450.13547474576734636No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTGTTTTTTTTCGTTTATC5440.13522616825217693No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGCGATTGTTTCGGAGGAGAGG5330.13249181558531306No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGCGGTGTTGAT5280.13124892800946583No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGTGGGGGGTAT5280.13124892800946583No Hit
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGGAAGGTGGGTA5040.1252830676453992No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGCGTTATTTTGTGATGGGTTAG5020.12478591261506032No Hit
GATTGTTTAGGGAGGGGGTTAATAGTGTGTTAGTATTGAGGTTAGGTTTG5010.12453733509989087No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTAGTTTTATTTTTAAATTT4790.11906862976616313No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGTGTGAAGGTT4620.1148428120082826No Hit
TGGTTTTTATTTTTGTGTTTTTTGGTTTTGTTAGGAGTGGGTAGTTAAGG4280.10639117649252154No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTCGT4200.10440255637116601No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTTTT4170.10365682382565766No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTAATTAATTGAGAAGTTGTTTT4110.10216535873464103No Hit
GAATTGAAGGAGAATTTATTTGTGGTTGTTATTGTTTTATTAGGATAGTT4100.10191678121947156No Hit
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGAGTTTAGGAAGGCGGGTA4050.10067389364362435No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)