FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402289
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC129943.2300162321117405No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG129813.226784724414538No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT126833.152708624894044No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA125733.125365098225405No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT113782.828314967597921No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA103692.577500254791953No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA93752.330414204713527No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC84442.098988538090776No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC84052.0892940149991674No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT83092.065430573542901No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC81902.0358498492377373No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC78751.9575479319593625No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT76871.9108153591075074No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA76711.906838118864796No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC73071.8163559033431191No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC70031.7407883387316085No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA68991.7149362771539864No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC68041.6913214132128893No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA64251.5971105349636705No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA62941.5645468804764733No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG52091.294840276517628No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC51221.2732140326978865No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA47611.1834775497217174No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA43391.0785778383202125No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT41101.02165358734641No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA38630.9602549410995578No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA37480.9316685268550718No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT33680.8372090710906835No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC31870.7922165408450144No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA31360.7795390875713728No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC31310.7782961999955257No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA27680.6880625619890178No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT27540.6845824767766456No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA27480.683091011685629No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA25330.6296468459241987No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT25010.6216923654387766No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC23590.5863943582847156No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA23330.5799313428903102No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC20460.5085895960366801No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA19730.4904434374293108No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA19260.47876029421634697No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT17930.44569948469881104No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA16770.4168644929391557No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC15790.39250389645255024No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT14000.34800852123722No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA13400.33309387032705345No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCA11960.2972987081426537No Hit
TAAAACTAAACCCCACATACCTTCCCCAACCCAAACACAACACTAACACA11720.29133284777858703No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT10120.25156044535147615No Hit
AATAAAAAACTCCCTCTTCCAAAAAATCATTTAAAATTCAAAACCTTATT10010.2488260926846123No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC9870.2453460074722401No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATGTCG8750.21750532577326248No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA8430.20955084528784035No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG7530.18717886892259047No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC7490.1861845588619127No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC7220.17947296595233775No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC6800.16903271031522116No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC6550.16281827243598507No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACAAAAAAAATAATCACA6540.16256969492081563No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC6500.16157538486013787No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC6410.1593381872236129No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA6290.15635525704157957No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACGCCAAACCCCCACAAAAC5990.14889793158649628No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCAAAACCCAAAAAA5910.14690931146514075No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA5830.14492069134378519No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC5800.14417495879827685No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT5690.141440606131413No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC5680.14119202861624355No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA5570.1384576759493797No Hit
CATAATCACCCACAACAAATTTCCCGCCACCGAAATAAACCGCCCCCTAA5470.13597190079768523No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA5380.13373470316116026No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT5370.1334861256459908No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC5160.12826599782743253No Hit
AAACTACAAAATCAAAACCAACCCCCTAATACCTTGTAGAACCATGTCGT5050.12553164516056864No Hit
ATACAAAACAAACCCCTATCTCCTCCAAACCCACAATTAATCAACACTCC4910.12205155994819644No Hit
CAAAACAAACAACACTAAAACCAAACTAATGAAAAACAAATCCCGCATCG4790.11906862976616313No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCGATCCACTC4690.1165828546144687No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4640.1153399670386215No Hit
CTCATTCAAAAAACCCAAAAAACCGAACCCAACTCATTCCACACCAACAT4590.11409707946277428No Hit
AAACTACAAAATCAAAACCAACCCGAAACCGCACCAAACCCCCACAAAAC4580.11384850194760482No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAAACCCCCACAAAAC4380.10887695164421597No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA4330.10763406406836876No Hit
AACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTAATTTATAC4310.10713690903802987No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAGCCCCCACCACCCGCAA4270.1061425989773521No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT4220.10489971140150488No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA4180.10390540134082712No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)