FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315119
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT118753.768417645397453No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA116073.683370409273957No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA109973.489792744963014No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG105103.3352479539475564No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT103983.2997058254183336No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA90562.8738349639342595No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT87952.7910091108438397No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC83422.6472538945604676No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT75842.4067098461216236No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC74442.362282185460096No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC72952.3149984608988987No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC72902.3134117587324154No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC71332.2635893107048446No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA63472.0141597301336955No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC62731.9906765380697449No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC60471.918957600144707No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA51221.6254176993453266No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC47271.5000682281931588No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC46501.4756330148293184No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA42201.3391766285117686No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC41571.319184181214081No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA40911.2982397126165037No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT40461.2839593931181554No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA35611.130049282969291No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA34061.0808615158083137No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA32001.0154893865492085No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC30980.9831206623529524No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT30600.9710617258876805No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC29060.9221912991599999No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT28710.9110843839946179No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA27530.8736382128656158No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT22860.7254402305160907No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA22310.7079865066847763No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA22010.6984662936858774No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACGCAAACAAACC18780.5959653337310666No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC17900.5680393756009635No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT14320.45443150048077074No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC13860.43983384054912583No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA11170.35446926399233303No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT10200.32368724196256016No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA9930.3151190502635512No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAATACCTAAAATAAA9690.3075028798644322No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCGCACTCCCTTCTAACACC9430.29925202859871985No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA9110.28909713473322773No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC8150.25863245313675154No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT7420.2354666015060977No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC6940.22023426070785956No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC6730.2135701116086304No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA5420.17199851484677217No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC5280.16755574878061938No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA5190.16469968488094971No Hit
TAAAACTAAACCCCACATACCTTCCAAATCATGGAACACAGCCAGTTCCT4870.15454479101545765No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4860.15422745058216103No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG4760.15105404624919475No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAGAAAAACCCAATTCACCCA4560.1447072375832622No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC4350.13804308848403302No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATAATAAA4240.1345523437177701No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAGCAT4090.1297922372183207No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA4080.12947489678502408No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA3930.12471479028557467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)