FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344994
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT150074.3499307234328715No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA142634.134274798982012No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT132903.8522409085375395No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG126063.653976590897233No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA123033.566148976503939No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC122663.5554241523040977No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG119503.4638283564351844No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT106033.073386783538264No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA100832.92265952451347No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC100172.9035287570218613No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC99662.888745891232891No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA93312.7046847191545362No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC91642.656278080198496No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA90872.6339588514582863No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA89292.5881609535238295No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC89232.5864217928427737No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA77632.2501840611720785No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA62271.8049589268219157No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC60201.7449578833255068No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT56261.6307529986028741No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA52261.514808953199186No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA46701.3536467300880595No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC43781.269007576943367No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA43411.258282752743526No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT42451.2304561818466409No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAC33740.9779880229801098No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA28650.8304492252039166No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA22100.6405908508553773No Hit
TACTATCCACACTCTCTTACCTCCCAAAATACTAAAATTACAAACGTAAA21840.6330544879041375No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC20160.5843579888345884No Hit
CATAATCACCCAATCTAAAACCAAAAACCTACTGTAGAACCATGTCGTCA16820.4875447109225088No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC15860.45971814002562367No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA15670.45421079786894847No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA14920.43247128935575685No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAACAACACAAA11650.3376870322382418No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC10000.2898601135092205No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT9710.28145417021745306No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA6830.19797445752679754No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG6080.17623494901360603No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAAACAAAAACACCTAACGCT5220.15130697925181308No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCCCAACAAA5100.14782865788970243No Hit
ATAATACTCCTTTATCCCAATCCCCCAAACCCTTCACCAAAAATACTAAA4890.14174159550600882No Hit
AATACCTTAACTCCCTAAAACCCAATATAAATCAAAAATATCCCAAAACT3690.10695838188490235No Hit
AAAACAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACAA3550.10290034029577326No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)