FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000888615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431560
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA171953.9843822411715637No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT163373.7855686347205486No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT156073.6164148669941607No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA141773.285058856242469No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT126662.9349337287978496No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG120432.7905737325053295No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC110432.5588562424691816No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC104842.429326165538975No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC102042.3644452683288533No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA101452.350773936416721No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC99212.2988692186486235No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA96262.23051255908796No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA95712.2177680971359717No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA95462.211975159885068No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA94162.181851886180369No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC87352.024052275465752No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC79831.8498007229585687No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG73241.6970988970247476No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC66711.545787376031143No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC54381.2600797108165724No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA47411.0985726202613773No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA47281.0955602928909074No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT43791.014690888868292No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA40210.9317360274353509No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT40030.9275651126147002No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT38070.8821484845676152No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC34660.8031328204652888No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC33600.7785707665214571No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA32470.7523866901473724No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACAACCCTCG31520.7303735285939383No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC31220.7234220038928538No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC29870.6921401427379739No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT29790.6902864028176847No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA29770.6898229678376124No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC22580.523218092501622No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA20820.48243581425526No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA18610.4312262489572713No Hit
ACTCTAAAAAACCAACTCCTCCAAAATACCCCTCTTAACTACCTTCCTTG18510.4289090740569098No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT17290.40063954027249976No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAACAATACCC17120.3967003429418852No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC16860.3906756882009454No Hit
TACTATCCACACTCTCTTACCTCCCTACTATCTCATTTACAACAAAAAAC14760.3420150152933543No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT13150.3047084993975345No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT10250.23751042728705163No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC10030.23241264250625637No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA9350.21665585318379832No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA6820.15803132820465288No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT6030.13972564649179722No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT6010.1392622115117249No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACA5960.13810362406154417No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA5920.1371767541013996No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA4540.10519974047641116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)