FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294047
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT93993.196427781953225No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA92063.1307920162423017No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG89793.0535934731522514No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC82992.822337925569722No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC77672.6414144677551548No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT75892.5808799273585517No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA70812.408118430046897No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA64452.191826476719708No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC61762.1003445027495604No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT61702.0983040126238324No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC60322.0513727397320833No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC57851.967372562889606No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC57391.9517288052590231No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT56861.9337044758150908No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA53161.807874251395185No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA53131.8068540063323208No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT52551.7871292684502815No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG49971.6993881930439694No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC49431.6810237819124152No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA43881.4922784452825568No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC43271.4715334623376535No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA39941.3582862603597383No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA32611.1090063833332766No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA31501.071257316007305No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA29721.0107227756107018No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA28450.9675324012827882No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA27820.946107254962642No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT27230.9260424353929814No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA26820.9120990862005053No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA24540.8345604614228338No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC24390.8294592361085132No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA23460.7978316391597262No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC22970.7811676364662792No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC21580.7338962818869093No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAAACCCCCACAAAAC21060.7162120341305982No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC19630.6675803528007427No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA18710.636292837539577No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT17210.585280584396372No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC14760.5019605709291373No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA13980.4754341992946706No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA13100.4455070107839903No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC13010.44244627559539806No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT12190.41455957721044595No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA11480.3904137773893289No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT11210.38123157182355205No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT11090.37715059157209563No Hit
AATACCTTAACTCCCTAAAACCCAAAATAACTAAAATAACTAAATTAACC11080.3768105098844743No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC10640.3618469156291341No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA10410.3540250368138427No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCACTCTAACCACCCTCG10300.35028413825000765No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC10030.3411019326842307No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT9990.3397416059337453No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA9820.33396021724418207No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA9070.30845409067257956No Hit
AAACTACAAAATCAAAACCAACCCAAAACCGCACCAAACCCCCACAAAAC8080.27478600359806427No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT7850.2669641247827728No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATACAAAACCACCAAACGCT7150.24315840664927715No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA7040.23941750808544213No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA6340.21561178995194644No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC6090.20710974776141228No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTGTCCTATAA5950.20234860413471314No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC5760.1958870520699072No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC5600.19044574506796533No Hit
AAAACAAAAAAAACTCCTAATCCCTCCTACGAATTTCTATATTCAAATAT5160.1754821508126252No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCACCG5040.1714011705611688No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT4950.16834043537257648No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG4760.16187888330777053No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCGATCCACTC4690.15949831149442095No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC4370.14861569749053724No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4350.1479355341152945No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT4260.14487479892670221No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAACAAACCACCCCCTAA4150.14113390036286716No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATCAATTTATAC3990.1356925933609253No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC3940.13399218492281845No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACACTACCTT3840.1305913680466048No Hit
ACTCCCACACACACTAACACAATAAAACAACACTACGAACATCAATACAC3760.12787071454563387No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA3660.12446989766942018No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT3610.12276948923131337No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA3440.11698810054175013No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC3320.11290712029029372No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA3200.10882614003883734No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA3040.10338483303689545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)