FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384919
Sequences flagged as poor quality0
Sequence length301
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAATATTGTTGTTTTTTAAGGTTTGTTCGTTATTTTACGATGAGTTAG231836.022825581485975No Hit
GTGAAGTTTTTGGTTTTTTGTTGTATAGATGAGGTTGGGATGTAAGGAGA169664.407680576952553No Hit
GATTGTTTAGGGAGGGGGTTAATAGCGTGTTAGTATTGAGGTTAGGTTTG166354.321688459130363No Hit
AGAAGGGTTAGTTATAGGGGAGGATGTGGTATAGGTGTTTATGGGATTTT157754.098264829743401No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTCGTGTAGAGGGTTCGTTGGGGTTT156904.076182261722596No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGTGTTGAT148583.8600328900366048No Hit
GTTGGGGATTGAGGTTTTTAGAGTAGAGGAGTTAGTTGTGGGTATTAGTG144573.755855127961987No Hit
GTTAAAAGAATTTGGAAGGGTTTGTGAGATATTGGATTGCGGGGTAATAA143023.715586915688755No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTCGGGTA141603.678696037348118No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTCGGAGGAGAGG118483.0780501871822383No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGACGTTGAGCGTGAAGAAGTGG116643.030247922290144No Hit
GTTGGTTTTTTTGGGGTGAAGCGGTTTTTATTTTCGTTGATGAACGGTTT109222.8374800932144164No Hit
GGTGGGGAATAAGGTTTTGTATTTATTGAGTTTTTGTTATAGGTTTGGGT108532.8195542438798813No Hit
GAATTGAAGGAGAATTTATTTGTGGTTGTTATCGTTTTATTAGGATAGTT95202.47324761833009No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTATGGGATAGGGAGTAT78052.0276993341456255No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGT77542.0144497933331427No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTT77372.010033279728982No Hit
GGTTGTAGGAGGTTTTTAGTAAGGGAGGGGTTGTAGGAGTGTTTTTGGGG77002.0004208677669846No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTATGTTGGAGT76761.9941857897375812No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTAAGGTATTGTGG74481.934952548458247No Hit
TGATTTTGTGAGTTTATAGGTTTAGGGTTTTAGTAGTAGAATTATTTTGG52581.3660016782751696No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTATGGGATAGGGAGTATT40521.0526890072976394No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTT33910.8809645665711487No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTAATTAATTGAGAAGTTGT26990.701186483390012No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTAATTAATTGAGAAGTTGTT26450.6871575578238538No Hit
GGAGTTGAGTGGTATAGTTGGTATTATTACGTATTAGTAGAGGTTTTTTG22390.5816808211597765No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTTATTTTTGGTTATTTTAGT21460.5575198937958376No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTAATTAATTGAGAAGTTGTTT18990.493350549076559No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTG18830.48919383039028985No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGGGTAGGTATTGTTAGTTTTATT18650.4845175218682372No Hit
GGGGATATTTAGGTTTGTTTTTTTTTGGGTTTGGTGTTTTTGGAGTTTTG12260.31850856933536664No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTTATTTTTGGGTTTTAGGGA10470.27200527903273153No Hit
TTTTTTTTGGGTTTTTAGGGTAGAGTTCGGGTTGGGGAGGTTAGTGTTTC9960.2587557382202489No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTGTTTT9620.2499227110119272No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTAATTAATTGAGAAGTTGTTTT9180.23849173462468726No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTT9090.2361535803636609No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTAATTAATTGAGAAGTTG8680.22550198873009644No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTTATGGGATAGGGAGTA7810.20289983087350846No Hit
TGATTTTGTGAGTTTATAGGTTTAGGTTTTAGTAGTAGAATTATTTTGGT7190.18679254596421585No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTAGGGTATTGTGG6620.17198423564438234No Hit
AGGTATTAGGGAGTTGTATGGGGTTGGTTTTGATTTTGTAGTTTAGACCA5790.15042125745936158No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTTGTGTAGAGGGTTCGTTGGGGTTT5030.13067684369958354No Hit
GATTGTTTAGGGAGGGGGTTAATAGCGTGTTAATATTGAGGTTAGGTTTG4830.12548094534174722No Hit
GTGAAGTTTTTGGTTTTTTGTTGTATAGATGAAGTTGGGATGTAAGGAGA4620.12002525206601909No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGAGTGTAGGTGGTGTTGAT4090.10625612141775281No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTCGTGTAAAGGGTTCGTTGGGGTTT4040.10495714682829374No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGAAGTGGGGGCGTCGGGTA3850.10002104338834923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)