FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384919
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA216545.625599152029388No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT214955.584291760084589No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT167884.36143708156781No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC162744.227902493771417No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA158434.115930884160044No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC141123.6662258812893103No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG139823.632452541963374No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT139483.6236195147550525No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA137813.5802337634671186No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA123133.1988548240019328No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA122463.1814485645031807No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC107932.8039665488063723No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC104262.7086218139400757No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA95982.493511621925652No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA82832.1518813048979135No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC78192.031336462996111No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA63421.6476193692698986No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA63151.6406049064868193No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC56781.4751155437897325No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT54031.403671941369483No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA51891.3480758289406343No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC46991.2207763191736443No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACTAAAAAATAAACCCCTA29460.7653558281092906No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC26980.7009266884721201No Hit
AATACCTTAACTCCCTAAAACCCAAAAATAACTAAAATAACCAAAAATAA20850.5416724038044367No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT20100.5221877849625506No Hit
AAAAAAATCCTTCCAACCAATAAAACTAACAATACCTACCCAACAAAACT18660.48477731678612906No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT13500.35072313915395187No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA12780.3320179050657411No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTAAAAAAAAAAACAACAT12730.33071893047628204No Hit
AATACCTTAACTCCCTAAAACCCAAAAATAACTAAATTAACCAAAAATAA11760.30551882344077586No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA11050.2870733842704569No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA9380.24368763298252358No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTAAAAAAAAAACAACATT7310.18991008497891765No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC7240.18809152055367492No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA5810.1509408472951452No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAACACACGACC5760.14964187270568613No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCCTCTATCCTATAA5480.14236761500471526No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCACCAAAAAAACC5160.13405417763217717No Hit
AAAACAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACAA4940.1283386894385572No Hit
CCCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA4250.11041284010402189No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTAAAAAAAAAAAACAACA4160.10807468584299554No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACTAAGAAATAAACCCCTA4070.1057365315819692No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)