FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333051
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG114533.4388126743351624No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT106423.1953064245415863No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA103353.103128349712206No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT95162.85722006539539No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC85262.5599682931442933No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT84482.536548456542692No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA83222.498716412801643No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA82372.4731947959922054No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC77602.32997348754395No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT77222.3185638235585544No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC77152.316462043350718No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC69262.079561388496056No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC67532.0276173919309652No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA60271.809632758946828No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA59741.7937192802303552No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC54421.6339839844348163No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC52751.5838415137621564No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA52001.5613224401067705No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC50451.5147830212189726No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA50021.501872085656551No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA49931.4991697968179047No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA47891.4379179164752545No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC47831.4361163905828236No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA45071.3532461995310028No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG42421.2736788059486386No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT39001.170991830080078No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT31800.9548087229883712No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA31480.9452005848954065No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA27110.8139894490633567No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA26400.7926713926695911No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC26080.7830632545766264No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC24910.7479334996742241No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT19910.59780634197165No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT18990.5701829449543764No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT18580.5578725180227653No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC18400.5524679403454726No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA18360.5512669230838521No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC17450.5239437803819835No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA17080.5128343707119931No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA16370.4915163143182275No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC14120.42395909335206916No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCGATCCACTC13380.4017402740120883No Hit
TACTATCCACACTCTCTTACCTCCCGACCCCCGCACTCCCTTCTAACACC11590.3479947515545667No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC11280.33868686777700713No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC11060.33208127283809385No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA9790.29394897478164006No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT9740.29244770320461433No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC9630.2891449057351577No Hit
CATAATCACCCACAACAAATTTCCCGCCGCCGAAATAAACCGCCCCCTAA9310.27953676764219293No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC9190.2759337158573312No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC8660.2600202371408583No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA8610.2585189655638326No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA8550.2567174396714017No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC7730.23209658580817952No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA7680.2305953142311538No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA7580.22759277107710232No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCAATCCACTC7170.21528234414549124No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT6920.20777598626036253No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT6470.19426454206713087No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA6350.19066149028226909No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCGATCCACTC6170.18525691260497643No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC6160.18495665828957125No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA6090.18285487808173523No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT5800.17414750293498593No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC5420.16273783894959032No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT5030.1510279206487895No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC4840.14532308865609173No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC4770.14322130844825567No Hit
TAAAACTAAACCCCACATACCTTCCAAATCATAAAACACAACCAATTCCT4770.14322130844825567No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAGAAAAATACCTAAAATAA4440.13331291603988576No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC4440.13331291603988576No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC4390.13181164446286003No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA4340.1303103728858343No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACGAACATTAAAACCTTCATC4320.129709864255024No Hit
TAAAACTAAACCCCACATACCTTCCCCAACCCAAACACAACACTAACACA4320.129709864255024No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG4290.12880910130880857No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4180.12550630383935193No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT4110.12340452363151588No Hit
CCAACATATACAAACACAAAATACTCAACGACTACCTGCGCGAACGAACC3920.11769969163881809No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCGATTCACCCA3630.10899231649206878No Hit
AAAAATCCTAAAAACCCCAAAACCACGAACTAACGAAAAAAATAATCACA3570.1071907905996379No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA3450.1035877388147761No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)