FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331289
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG115213.477628294329121No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT114073.443217251402854No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT103463.1229530711855813No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA93792.8310629088197916No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC86122.599542997201839No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT76342.3043324710449187No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC73362.214380797430642No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT72352.1838938208029846No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA69862.1087328586219285No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA65951.9907090184099079No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA65231.9689757281406868No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA64801.9559961242299018No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC59581.798429769778049No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC58361.7616039168218685No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC57061.7223632538357747No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA54501.645089332878544No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA53991.6296949189378458No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCCATTAACCTATCCC49561.495974813531388No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA49161.4839007633818206No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC48161.4537156380079024No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG48081.4513008279779889No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA44961.3571232368113642No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC39461.1911050472548137No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA38541.163334731910809No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC35901.0836460009236648No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA34311.0356516515791347No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA30880.9321166715465953No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA28610.8635964369478009No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA27060.8168094926182276No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC26430.7977928636326591No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT24330.7344041003474308No Hit
TACTATCCACACTCTCTTACCTCCCGACCCCCACACTCCCTTCTAACACC22930.6921449248239453No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA22750.68671160225664No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC22380.6755431058682903No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA22110.6673931220173324No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC22050.6655820144948972No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA21610.6523005593303732No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA18430.5563118606413132No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC18260.5511803893277472No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT17990.5430304054767892No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC17740.5354841241333096No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT17590.5309563553272219No Hit
ATACAAAACAAACCCCTATCTCCTCTGCCGCGACCTTAAAAAACAATACC16990.5128452801028709No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCAAAACCCAAAAAA14990.45247502935503436No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT14770.4458343017727724No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA14620.44130653296668465No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA14290.4313454415932917No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT13990.42228990398111615No Hit
CATAATCACCCACAACAAATTTCCCGCCACCAAAATAAACCACCCCCTAA13470.40659363878667876No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC13460.4062917875329395No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCGATCCACTC13330.4023677212343302No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA12790.3860677535324143No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC12730.3842566460099792No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC10910.329319717829448No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC10840.32720675905327373No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC10740.3241882465158819No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT10240.3090956838289228No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA10130.3057753200377918No Hit
CCCAAAAATTAATAAATAAACCACTCACCTACTTAATCTCCCTGTAGAAC9900.2988327412017906No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT9400.28374017851483146No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT7450.2248791840356909No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC7410.22367177902073415No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC7090.21401253890108032No Hit
CCATAAAAACAAAAACCTAAAAAAAAATCTTACCCCTCTCTAAACCAAAA6930.20918291884125342No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT6890.20797551382629667No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT6840.20646625755760076No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT6810.20556070379638322No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC5710.17235706588507316No Hit
TAAAACTAAACCCCACATACCTTCCCCAACCCAAACACAACACTAACACA5280.15937746197428831No Hit
AATACCTTAACTCCCTAAAACCCAAAATAACTAAATTAACCAAAAATAAC4350.13130529537654434No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4030.12164605525689051No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCAAAAAATAAACCCCTA3930.1186275427194987No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA3890.11742013770454196No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCCCAAAACGACCATCAAACA3670.11077941012227994No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCGCC3650.11017570761480157No Hit
ACTCCCACACACACTAACACAATAAAACAACACTACGAACATCAATACAC3590.10836460009236648No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC3510.10594979006245302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)