FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270861
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGGAAGGCGGGTA108073.989869342577927No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTTCGTTATTTTACGATGAGTTAG98083.621045480892413No Hit
GATTGTTTAGGGAGGGGGTTAATAGCGTGTTAGTATTGAGGTTAGGTTTG90653.3467350412204047No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTCGGAAGTATGGTATTT84043.1026984320370965No Hit
TGATTTTGTGAGTTTATAGGTTTAGGTTTTAGTAGTAGAATTATTTTGGT80622.9764344073159297No Hit
AGAAGGGTTAGTTATAGGGGAGGATGTGGTATAGGTGTTTATGGGATTTT74272.741996817555868No Hit
GTTGGGGATTGAGGTTTTTAGAGTAGAGGAGTTAGTTGTGGGTATTAGTG72482.6759112607573625No Hit
GTGAAGTTTTTGGTTTTTTGTTGTATAGATGAGGTTGGGATGTAAGGAGA72172.664466276060415No Hit
GTTAAAAGAATTTGGAAGGGTTTGTGAGATATTGGATTGCGGGGTAATAA72062.660405152458272No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTTGTTTATC67882.5060824555768457No Hit
GGTGGGGAATAAGGTTTTGTATTTATTGAGTTTTTGTTATAGGTTTGGGT66402.451441883475288No Hit
TGGTTTTTATTTTTGTGTTTTTTGGGGATGGTTTTTAAGGATAGTATGTT63062.3281314031920433No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGGTTTTTTGT60182.22180380342685No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTCGGAGGAGAGG59062.1804541812959415No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTCGGGTA54131.9984420053089962No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGTGGGGGGTAT52301.9308796762915297No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTCGTGTAGAGGGTTCGTTGGGGTTT51041.8843613513942574No Hit
GAATTGAAGGAGAATTTATTTGTGGTTGTTATCGTTTTATTAGGATAGTT43091.5908528728757556No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTATTTTTGATTTTTTTGATT40961.5122147522160814No Hit
GATAGGTTTGGAGATAGTTTTGTTTAAAGATATTTAGTATTTTAGTGGTA38341.4154861718741347No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGT36591.35087738729459No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGTGTTGAT36231.337586437323941No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTTGTGTAGAGGGTTTGTTGGGGTTT35141.2973443943572533No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTTGTTTGAGGTTTTTTGT34901.2884837610434872No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTATGGGATAGGGAGTAT34701.2810998999486822No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTAAGGTATTGTGG33991.2548871930621242No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGTGTGAAGAAGTGG33371.2319972236682284No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGCAGGTGGTGTTGAT33261.2279361000660856No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTATGTTGGAGT31331.1566818405012165No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTAGTTTTATTTTTAAATT28521.052938592119205No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTT26870.9920217380870631No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGTGTTTATTTTTTTT23030.8502516050668054No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTAATTAATTGAGAAGTTGT19110.7055279276086258No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTAATTAATTGAGAAGTTGTT16890.6235670694562894No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTGTTTATCG16510.6095377333761597No Hit
GGGGTGTATTTATTAGTTTTAGGGATGTATTTTTAGGTTTCGGGGGTTTA16240.5995695208981728No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGCGTGAAGAAGTGG15690.5792639028874589No Hit
AGGTATTAGGGAGTTGTATGGGGTTGGTTTTGATTTTGTAGTTTAGACCA14380.5308996127164856No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGCGTGAAGGTT12630.46629082813694106No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTG12190.4500463337283699No Hit
TTTGGAGAGGAGGTTTTTAGATTTTATTTTGGTGGTATAGCGTTTTTTTT11940.44081650735986355No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGTGTGAAGGTT11090.4094350977069419No Hit
TAGGTTGTTTTGGTTTAGAGAGGGCGGGAGTTTTTGTAGGATAATATAGT10910.40278962272161734No Hit
TGTTGGGTAGGTATTGTTAGTTTTATTGGTTGGAAGGATTTTTTTAGACC10300.3802688463824619No Hit
AATTTGTTGTGGGTGATTATGGTGTACGGAGTTAGTGTGGATTTGTGTGG10060.37140821306869576No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTT9560.35294856033168304No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTAATTAATTGAGAAGTTGTTT9470.3496258228390208No Hit
AATTTGTTGTGGGTGATTATGGTGTATGGAGTTAGTGTGGATTTGTGTGG8750.3230439228977225No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTTGGGTA8280.30569184932493054No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATACGTTGTTGGTTGTTTTGA8180.30199991877752796No Hit
GTTTATGAGTAGGTTTTTTTGGGGTTGTTGTATTGTGTTATGGATGTTTT7780.2872321965879178No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTATGGGATAGGGAGTATT6180.22816130782947713No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTAATTAATTGAGAAGTTG6100.22520776339155507No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTT5820.21487035785882797No Hit
TTTTTAGGTGAGATTAGGGATTTGGGTGTGGGGGGCGGTTAAAGGTATTT5400.1993642495597373No Hit
TTTTTAGGTGAGATTAGGGATTTGGGCGTGGGGGGCGGTTAAAGGTATTT5390.19899505650499702No Hit
TGGGTTGTGATTAGATGGTAGAAAGGGGTTTTAGTTAGGTTGATAATGGT5200.1919803884649322No Hit
TAGGTTGTTTTGGTTTAGAGAGGGTTTTTTGTTATAGAGAGTTAAGGGTT4850.1790586315490233No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTTTGTTTAT4220.15579946910038728No Hit
TTTGGAGAGGAGGTTTTTAGATTTTATTTTGGTGGTATAGTGTTTTTTTT4190.15469188993616653No Hit
TTTTTTTTGGGTTTTTAGGGTAGAGTTCGGGTTGGGGAGGTTAGTGTTTC3690.1362322371991538No Hit
TGATTTTGTGAGTTTATAGGTTTAGGTTAGAGATAGTGTTATTGGTTGGA3340.1233104802832449No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTAGTTTTATTTTTAAATTT3340.1233104802832449No Hit
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGAGTTTAGGAAGGCGGGTA3310.12220290111902415No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTAATTAATTGAGAAGTTGTTTT3250.11998774279058262No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTTATGGGATAGGGAGTA2830.10448163449149193No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTTGTGTAGAGGGTTCGTTGGGGTTT2790.10300486227253092No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTTAGTTTTATTTTTAAAT2760.10189728310831016No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)