FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354705
Sequences flagged as poor quality0
Sequence length301
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG144394.070706643548864No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT139273.926361342524069No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA120943.40959388787866No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC112653.1758785469615596No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA108383.0554968213022087No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT103482.9173538574308227No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC102012.875910968269407No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT100272.826856119874262No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC85612.41355492592436No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA84052.3695747170183674No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC78442.2114151196064333No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC71512.0160414992740447No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA64781.826306367262937No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC59081.6656094501064267No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT53241.5009655911250193No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA52231.4724912251025501No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC51401.4490914985692336No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC50471.4228725278752767No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT50181.414696719809419No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG49611.398627028093768No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA49041.3825573363781172No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT47751.3461890867058541No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA47501.3391409763042528No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC44001.2404674306818342No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA43721.2325735470320407No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA38611.0885101704233096No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA38001.0713127810434022No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC36031.0157736710787837No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA34740.979405421406521No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT32830.9255578579382867No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA31470.8872161373535754No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC31110.8770668583752697No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA29880.842390155199391No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT27420.773036748847634No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA25010.7050929645761971No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC23430.6605489068380768No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT23170.6532188720204113No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA21960.619106017676661No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC21920.6179783200124047No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT21590.6086748142822909No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC19390.5466514427481992No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCAATCCACTC17220.48547384446229963No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA16520.46573913533781597No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACGAACATTAAAACCTTCATC15800.44544057738120413No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT15600.4398020890599231No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT15070.4248600950085282No Hit
CCCAAAAATTAATAAATAAACCCCAAAAATTAATAAATAAACCACTCACC12730.3588897816495398No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC12170.34310201434995274No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACGCC11640.32816002029855795No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC11630.3278780958824939No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATCTCTAAA10230.28840867763352646No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA7930.2235660619387942No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC7910.2230022131066661No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC7220.20354942839824644No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC6840.19283630058781243No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG6810.19199052733962024No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC6110.17225581821513652No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC5890.16605348106172735No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA5820.16408001014927898No Hit
AAAAATCCTAAAAACCCCAAAACACAAATTCCAAAATCTACCTAATGTAG5210.14688262076937172No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC5180.14603684752117957No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC4910.13842488828745014No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4830.1361694929589377No Hit
ATACAAAACAAACCCCTATCTCCTCCACCGCGACCTTAAAAAACAATACC4420.12461059190031153No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA3910.11023244668104482No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA3880.10938667343285266No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTACACCAAAAATACTAAA3610.10177471419912322No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)