FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000888631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433447
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT130413.0086723405629754No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA119822.7643518123323036No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC113412.616467526594947No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT110582.5511769605049754No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT106492.4568170964385496No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA94662.1838886876596217No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA94032.1693540386713948No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT92032.1232122958516264No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA91732.116291034428661No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG90082.078224096602353No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC86081.9859406109628168No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC79091.8246752198077274No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC76641.7681515848535116No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC75201.7349295300232783No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA70901.6357247829607773No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC69931.6133460376931898No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA67171.54967043260191No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC67161.549439723887811No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC66701.5388271230392643No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA64881.4968381370732755No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA64131.4795349835158624No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT62401.439622375976763No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA61131.4103223692862104No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC55761.2864317898151332No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC55391.277895567393476No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA54961.267975092687226No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA50601.1673860933401317No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTGCTGGATACACGACC49191.134856164652195No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA43911.0130419636080075No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT40790.9410608448091693No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA40110.9253726522504481No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC37610.8676954737257381No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA37350.8616970471591682No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT34820.8033277424921617No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA33760.7788726187976847No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT31790.733423002120213No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC28510.6577505438957935No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA24950.5756182416766064No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC21820.5034064141636694No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA20630.4759520771859074No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT18750.4325788389353254No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC18020.4157371028061101No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCAAAAAATAAACCCCTA17770.40996938495363905No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATGCC16950.39105127039753423No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCACTCTAACCACCCTCG15740.3631355159915745No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC15670.3615205549928826No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA15120.34883157571744644No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG11440.2639307689290732No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT10660.2459354892293637No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC9720.2242488701040727No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC9020.2080992601171539No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC8410.19402602855712464No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA7660.17672287499971162No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT6970.16080397372689165No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAACAACACAAA6310.14557719859636817No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA6290.1451157811681705No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC6260.14442365502587398No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTGGA6250.14419294631177515No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA6080.14027089817209487No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA5160.11904569647500157No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA4810.11097089148154216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)