FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380953
Sequences flagged as poor quality0
Sequence length301
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT175464.605817515546537No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA163584.293968022301964No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA146013.832756271771058No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT134363.5269442687155634No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT132343.4739193548810485No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT129113.3891319926605123No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT129053.387556995219883No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA121233.182282328791216No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC107602.824495410194958No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA106942.8071704383480376No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC103152.707683100014963No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA99902.6223707386475494No Hit
AAACTACAAAATCAAAACCAACCCAAAACCGCACCAAACCCCCGCAAAAC85552.2456838507637427No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA73961.9414468451488767No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA66901.7561221463015122No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT64351.689184755074773No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA61111.6041348932807984No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC58891.5458599879775194No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA58731.5416599948025085No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA58691.5406099965087556No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC56101.4726226069882635No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA55291.4513601415397699No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT50641.3292978398910102No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA47041.2347979934532607No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA45671.198835551892228No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG40741.0694232621871989No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC39961.04894829545902No Hit
CAACCATAAATACACAAAAAACAACCATCACCCACTAACTCCAAATTCAA36320.9533984507275175No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA32080.8420986315897238No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC31530.8276611550506231No Hit
CAACCATAAATACACAAAAAACAACCTAAACCCCGAAAATAACTACTAAA30380.7974737041052308No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCGCC21600.5669990786264972No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA17950.47118673432155667No Hit
TAAAACTAAACCCCACATACCTTCCCCAACCCAAACACAACACTAACACA17490.45911175394339987No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT16540.43417429446677147No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC12180.3197244804477193No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCACAACCACGCTACCTT11980.3144744889789554No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC11700.307124500922686No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAGCCCCCACCACCCGCAA11670.3063370022023714No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA8780.2304746254787336No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA8650.22706213102403708No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAG8330.2186621446740149No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA7790.20448716770835249No Hit
AATACCTTAACTCCCTAAAACCCAAAATAACTAAATTAACCAAAAATAAC6880.1805997065254769No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA4700.12337479951595079No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA4610.12101230335500705No Hit
AAAACAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACAA4410.11576231188624318No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA4280.11234981743154666No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)