FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292365
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG85702.9312674225711013No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA85202.9141655122877226No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT84422.887486532245652No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT80722.76093239614865No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC78072.670292271646743No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC71982.461991004395191No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA71352.440442597438134No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT71182.434627947941785No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT64352.2010158534708326No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC61732.1114018435859285No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA59132.0224719101123596No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC54991.8808680929659842No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA53651.8350349734065297No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC53621.834008858789527No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC52221.7861235099960664No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC50821.7382381612026063No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC49601.6965095001111625No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA49451.6913789270261488No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA49251.6845381629127976No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA47641.6294700118003183No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA43301.4810254305405914No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA41291.4122757512014092No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT40961.4009884904143792No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA36061.2333897696372684No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG34341.174559198262446No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC33501.1458279889863698No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC30971.0592923229524738No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA30011.0264566552083867No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA29881.0220101585347083No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC26790.9163203529834283No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA24570.840387871325227No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA24540.8393617567082243No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC23720.8113146238434833No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA20510.7015203598241924No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT20290.6939955192995058No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT17300.5917260958049014No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT16400.5609426572948198No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA15540.5315273716074085No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA14850.507926735416346No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC14350.4908248251329674No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC14040.48022164075727264No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT13960.47748533511193203No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA13340.45627896636054244No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC11890.4066834265387444No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA11880.4063413883330768No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTGTT11650.3984745096027227No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC8880.3037299266328049No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATGTCG8640.29552100969678313No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC8500.29073247481743714No Hit
AAACTACAAAATCAAAACCAACCCAAAACCGCACCAGACCCCCACAAAAC8470.2897063602004344No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA7850.26849999144904485No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT7770.26576368580370424No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC7760.2654216475980367No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT7710.2637114565696988No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT7350.2513980811656662No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA6540.22369298650659278No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC6300.21548406957057104No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACGT6290.21514203136490345No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA6140.21001145827988987No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCAACACTCCCTTCTAACACC6120.2093273818685547No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC5860.20043438852119783No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAGATAAACCCCTA5830.19940827390419508No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA5590.19119935696817336No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA5540.18948916593983547No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCG4840.16554649154310536No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA4750.16246814769209722No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG4560.15596942178441334No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT4440.15186496331640245No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC4320.14776050484839157No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA4290.14673439023138884No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA4170.142629931763378No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA3950.13510509123869135No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC3940.1347630530330238No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT3850.13168470918201564No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAGAACGACCATCAAACA3600.12313375404032631No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC3580.12244967762899116No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA3160.10808407299095309No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA3100.10603184375694766No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCGCC3090.10568980555128009No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCAGACTCCCTTCTAACACC2970.1015853470832692No Hit
CAACTACCAAACCCTAAAAAACCACTCTACACTAACCGAAACCCAAAAAA2950.10090127067193405No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA2940.10055923246626648No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)