FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381663
Sequences flagged as poor quality0
Sequence length301
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT125743.294529467095317No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA111922.932429918540702No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT111312.9164472322441526No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC98552.582120876270427No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA97522.555133717441827No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG93792.457403520907188No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC93182.4414208346106383No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC93042.4377526770999545No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT87462.2915503991741404No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA80722.114954816159806No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA75801.986045280784357No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA75591.9805430445183316No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA74541.953031863188205No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC73951.937573199393182No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA69151.8118077990268902No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC66641.7460429750853501No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT63941.6752999373793112No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA57961.5186172094229726No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA57391.5036825681294754No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC57011.4937261406004774No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA56651.4842937355730055No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA48851.2799249599777816No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC45321.1874349884584043No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA43561.1413210083240974No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG43371.1363427945595983No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT41111.0771282518871361No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC37340.9783500103494445No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC37170.9738958190864715No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCG34310.8989606013682228No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT34250.8973885338636441No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC33090.8669952287751236No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC32630.8549427112400207No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA31920.8363399124358399No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT31740.831623709922104No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA27460.719482894595494No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT26310.6893516007577366No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT24750.6484778456386917No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA24440.6403554968650355No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT24080.6309230918375635No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC23970.6280409680791693No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA22780.5968616292383595No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCGATCCACTC22150.5803549204402837No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT18830.49336718518693196No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAACATCCCAAAAC16380.4291744287499706No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC16130.4226241474808929No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC15810.4142397874564734No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC15360.4024492811721335No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAGCA14310.3749380998420072No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT12410.32515596219701676No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA11410.298954837120706No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCACCT10670.27956600456423597No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC10570.2769458920566049No Hit
AAAAAAAATCCCAAAACAAACATACGAAGTCAAAAAAATACCTAAAATAA9670.2533648794879252No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT9460.24786264322189996No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAACAACAATACC8550.22401961940245715No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA8250.21615928187956387No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA7100.1860279880418065No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC7050.18471793178799098No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC6550.1716173692498356No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA6360.16663915548533653No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA6030.15799278421015397No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG5090.13336372663842183No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA4860.12733746787087036No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA4740.1241933328617131No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC4360.11423690533271498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)