FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000888639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411870
Sequences flagged as poor quality0
Sequence length301
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT138783.369509796780538No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA129163.1359409522422124No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA106732.591351640080608No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG102952.4995751086507876No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC99992.427707771869765No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT98702.3963872095564134No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT92982.2575084371282204No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA91912.231529366062107No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT88082.1385388593488237No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA84512.051861024109549No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC81921.988977104426154No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC79931.9406608881443175No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC79771.936776167237235No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC77731.887245975671935No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC75351.8294607521790858No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA73421.7826013062374049No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC70191.7041785029256806No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA61551.4944035739432344No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT59331.4405030713574671No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA59001.4324908344866099No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC53641.3023526840993516No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA53401.2965256027387284No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC50571.2278146016947096No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA46251.1229271372034866No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC44031.069026634617719No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA41010.9957025274965402No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG40800.9906038313059947No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA40140.97457935756428No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA36200.8789181052273776No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC35520.8624080413722776No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC33560.814820210260519No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT32550.7902979095345619No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA31380.7618908879015224No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC31260.7589773472212106No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT28530.6926942967441183No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA28510.692208706630733No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA27690.672299511981936No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA25580.6210697550197878No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA24110.5853788816859689No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT23720.5759098744749557No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC23520.5710539733411027No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT21360.5186102410954913No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA19570.4751499259475077No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT18340.4452861339743123No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC17120.4156651370578095No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA15680.3807026488940685No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC15190.3688056911161289No Hit
AAACTACAAAATCAAAACCAACCCAAAACCGCACCAAACCCCCGCAAAAC14110.3425838249933231No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA13460.3268021463083011No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTGAAAATTTCTAAA13360.3243741957413747No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC12840.3117488527933571No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT12740.30932090222643066No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA12490.3032510258091145No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCCA11470.27848593002646466No Hit
CATAATCACCCACAACAAATTTCCCACCGCCAAAATAAACCGCCCCCTAA11160.27095928326899266No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT10220.24813654793988393No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCG9680.23502561487848106No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC9370.2274989681210091No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC9020.21900114113676647No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC8630.2095321339257533No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC8360.20297666739505182No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA7700.1869521936533372No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA7080.17189890013839318No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCA7030.17068492485492995No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA6890.16728579406123292No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA6620.16073032753053149No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC6290.15271809065967418No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC6230.1512613203195183No Hit
AACCACTCACCTACTTAATCTCCCAATTCCAATATATCCAAAACTTACCC6020.14616262412897274No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC5780.14033554276834923No Hit
CCCAAAAATTAATAAATAAACCACTCACCTACTTAATCTCCCTGTAGAAC5760.13984995265496394No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACACTACCTT5730.139121567484886No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA5720.13887877242819335No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCACTCTAACCACCCTCG5380.1306237405006434No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG5380.1306237405006434No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT5340.1296525602738728No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA5040.12236870857309345No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC4620.11217131619200232No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAGCACCTATTAACCTATCCC4570.11095734090853909No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA4560.11071454585184647No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA4280.10391628426445237No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGGT4230.10270230898098914No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA4210.10221671886760386No Hit
CATAATCACCCACAACAAATTTCCCGCCACCAAAATAAACCACCCCCTAA4120.10003156335737004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)