FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000888641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414597
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT134353.240496192688321No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA119352.8786990740405742No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG105552.5458457248846473No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC100182.4163223564087533No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC96892.3369681883853475No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA91482.2064800275930603No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT91362.2035856506438782No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA82671.9939845199072836No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC80261.9358557828445453No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT77581.8712146976461481No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA75891.8304522222785016No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC72061.738073357983777No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC71721.7298726232944281No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC71351.7209482943677836No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT70951.7113003712038437No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA70591.7026172403562978No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC70321.6961048922206383No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC69411.6741558670226748No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA68661.6560660110902874No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT67341.6242278646492858No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA66451.6027612356095196No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA63611.534260981145546No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC47451.1444848853223732No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA47211.1386961314240094No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG42951.035945749728049No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT42271.0195442803493513No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC41481.0004896321005698No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA40100.9672042971849772No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA37910.9143819178624061No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC33710.813078724641037No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA31700.7645979107422388No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA31220.753020402945511No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT30940.746266856730753No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCG29180.7038159948094173No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC28660.6912736946962954No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT28510.687655723509818No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA21970.5299121797794002No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA20420.4925264775191331No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA20420.4925264775191331No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA18490.44597524825312296No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT18320.44187488090844845No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC17260.41630788452400763No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC16980.4095543383092497No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA16900.40762475367646167No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT16840.40617756520187076No Hit
AAAAAAAATCCCAAAACAAACATACAAAATCAAAAAAATACCTAAAATAA15830.3818165592129224No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC15440.372409834128081No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA14190.34226007424076876No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC12990.313316304748949No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT11910.2872669122063112No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA11770.28389013909893224No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC11500.27737779096327275No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAAACCCCCACAAAAC11340.2735186216976968No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTCCACCAAAAATACTAAA10150.24481605028497552No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC9720.2344445328837401No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA9540.23010296745996714No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA9410.22696739243168668No Hit
AAACTACAAAATCAAAACCAACCCGAAACCGCGCCAAACCCCCACAAAAC8590.20718914994560983No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA8530.20574196147101886No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCAAAACCCAAAAAA8030.19368205751609394No Hit
AAACTACAAAATCAAAACCAACCCGAAACCGCACCAAACCCCCACAAAAC7790.18789330361773No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC7470.18017496508657807No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA7330.17679819197919908No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC7080.17076824000173663No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG6780.1635322976287817No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT6570.15846713796771322No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA6340.15291958214844778No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT6270.15123119559475828No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT6170.1488192148037733No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA5860.14134207435171986No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT5800.13989488587712887No Hit
CCAACATATACAAACACAAAATACTCAACGACTACCTACGCAAACAAACC5460.13169415118777994No Hit
CAACTACCAAACCCTAAAAAACCACTCTACACTAACCGAAACCCAAAAAA5310.12807618000130247No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC4920.11866945491646105No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACACTACCTT4780.11529268180908206No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA4590.11070991830621062No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA4550.1097451259898166No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCGCC4340.10467996632874815No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTACACACCAACAT4250.10250918361686166No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)