FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309065
Sequences flagged as poor quality0
Sequence length301
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGGAAGGCGGGTA92572.9951628298254414No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTATC87622.835002345784867No Hit
GATTGTTTAGGGAGGGGGTTAATAGCGTGTTAGTATTGAGGTTAGGTTTG82562.671282739876725No Hit
TGATTTTGTGAGTTTATAGGTTTAGGTTTTAGTAGTAGAATTATTTTGGT75142.4312037920825715No Hit
GGTGGGGAATAAGGTTTTGTATTTATTGAGTTTTTGTTATAGGTTTGGGT74722.417614417679129No Hit
TAGGTTGTTTTGGTTTAGAGAGGGCGGGAGTTTTTGTAGGATAATATAGT72202.3360781712584733No Hit
AGAAGGGTTAGTTATAGGGGAGGATGTGGTATAGGTGTTTATGGGATTTT72142.3341368320579816No Hit
GTGAAGTTTTTGGTTTTTTGTTGTATAGATGAGGTTGGGATGTAAGGAGA69162.2377169851002217No Hit
GGTTGTAGGAGGTTTTTAGTAAGGGAGGGGTTGTAGGAGTGTTTTTGGGG67512.184330157086697No Hit
GTTGGGGATTGAGGTTTTTAGAGTAGAGGAGTTAGTTGTGGGTATTAGTG67172.1733292349505766No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGGTTTTTTGT67122.1717114522835No Hit
TGGTTTTTATTTTTGTGTTTTTTGGGGATGGTTTTTAAGGATAGTATGTT66232.1429149208095386No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTCGGGTA53251.7229385404364779No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTCGGAAGTATGGTATTT52931.712584731367188No Hit
GTTAAAAGAATTTGGAAGGGTTTGTGAGATATTGGATTGCGGGGTAATAA52791.7080549398993738No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTCGTGTAGAGGGTTCGTTGGGGTTT41861.35440764887645No Hit
GAATTGAAGGAGAATTTATTTGTGGTTGTTATCGTTTTATTAGGATAGTT41121.3304644654037177No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGTGTTATTTTGTGATGGGTTAG40561.3123452995324607No Hit
GATAGGTTTGGAGATAGTTTTGTTTAAAGATATTTAGTATTTTAGTGGTA37811.2233672528432529No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTCGGAGGAGAGG36731.1884231472344005No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTATGGGATAGGGAGTAT36091.1677155290958212No Hit
GTTGGTTTTTTTGGGGTGAAGCGGTTTTTATTTTCGTTGATGAACGGTTT35291.1418310064225974No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGT35081.1350363192208759No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGTGTTGAT34711.1230647274845098No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTAAGGTATTGTGG33621.087797065342242No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTATTTTTGATTTTTTTGATT32741.0593240904016954No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTT29430.9522268778412307No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTTCGTTATTTTACGATGAGTTAG29050.9399317295714493No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGTGTGAAGAAGTGG28240.9137236503648101No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTTGTGTAGAGGGTTCGTTGGGGTTT28070.9082231892967498No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTAGTTTTATTTTTAAATT26880.869719961820329No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTATGTTGGAGT24760.8011259767362852No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGCGTGAAGAAGTGG24540.7940077330011486No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTTGGAAGTATGGTATTT23220.7512982705903288No Hit
GGGGTGTATTTATTAGTTTTAGGGATGTATTTTTAGGTTTCGGGGGTTTA21510.6959701033763125No Hit
GGAGTTGAGTGGTATAGTTGGTATTATTACGTATTAGTAGAGGTTTTTTG20960.6781744940384709No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGTGTTTATTTTTTTT20520.6639380065681977No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGCGTGAAGGTT20220.6542313105657386No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTTGTTTGAGGTTTTTTGT18980.6141103004222412No Hit
AATTTGTTGTGGGTGATTATGGTGTATGGAGTTAGTGTGGATTTGTGTGG18440.596638247617815No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTAATTAATTGAGAAGTTGT17670.5717243945448368No Hit
TTTGGAGAGGAGGTTTTTAGATTTTATTTTGGTGGTATAGTGTTTTTTTT16460.5325740540015854No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGCGGGGGGTAT16440.5319269409347549No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTACGTTGGAGT15890.5141313315969133No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGTGGGGGGTAT15860.5131606619966674No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGCGGGGGGTAT15690.5076602009286072No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTAATTAATTGAGAAGTTGTT15650.506365974794946No Hit
TTTTTAGGTGAGATTAGGGATTTGGGCGTGGGGGGCGGTTAAAGGTATTT15000.4853348001229515No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGTGTGAAGGTT14800.4788636694546455No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTTGGTGATTGTTTTGGAGGAGAGG14160.4581560513160662No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGCGGGGGGTAT14120.45686182518240503No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTCGGGGCGTAGGCGGCGTTGAT13610.44036044197822466No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAC12680.4102696843706017No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGTGGGGGGTAT11720.37920825716273276No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTT11640.37661980489541036No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATACGTTGTTGGTTGTTTTGA11250.3640011000922136No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGCGGTGTTGAT10040.3248507595489622No Hit
GGGGATATTTAGGTTGTAAGATAGAGTGGGGTTTTAGACCAAGTCTCTGC9110.2947600019413392No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTG8970.290230210473525No Hit
GATTGTTTAGGGAGGGGGTTAATAGTGTGTTAGTATTGAGGTTAGGTTTG8960.28990665394010967No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGTTT8740.2827884102049731No Hit
TTTAAGGTTTGTGTTGATGTTAATGTTATAGGTTATGATAAGGTATGTTA8710.28181774060472714No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTTGTGATGGGTTAG8710.28181774060472714No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTAATTAATTGAGAAGTTGTTT8360.27049326193519163No Hit
TTTAAGGTTTGTGTTGATGTTAATGTTATAGGTTACGATAAGGTATGTTA7860.25431543526442657No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTATGGGATAGGGAGTATT7520.24331451312830632No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTTTT7170.23199003445877078No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTTGGGTA6700.21678287738825167No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTCGTGTAGAGGGTTTGTTGGGGTTT6660.21548865125459046No Hit
TGGGTTGTGATTAGATGGTAGAAAGGGGTTTTAGTTAGGTTGATAATGGT6380.20642906831896202No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTATT6010.1944574765825959No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGCGTTATTTTGTGATGGGTTAG5790.1873392328474593No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGCGTTTATTTTTTTT5780.18701567631404398No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTAATTAATTGAGAAGTTG5740.18572145018038277No Hit
GTTTATGAGTAGGTTTTTTTGGGGTTGTTGTATTGTGTTATGGATGTTTT5680.18378011097989094No Hit
GTTGGTTTTTTTGGGGTGAAGTGGTTTTTATTTTCGTTGATGAACGGTTT5470.17698542377816964No Hit
GTTGGGGATTGAGGTTTTTAGAGTAGAGGAGTTGGTTGTGGGTATTAGTG5180.1676022843091259No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGTGTTGGGTA5040.1630724928413117No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTCGTTTATCG5030.1627489363078964No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTAGGGTATTGTGG4980.1611311536408199No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGTGGGGGGTAT4810.15563069257275977No Hit
GGGGATATTTAGGTTTGTTTTTTTTTGGGTTTGGTGTTTTTGGAGTTTTG4650.15045378803811496No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTCGCGATGGGTTAG4380.14171776163590183No Hit
TAGGTTGTTTTGGTTTAGAGAGGGTGGGAGTTTTTGTAGGATAATATAGT4340.14042353550224063No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTCGGGGCGTAGGTGGTGTTGAT4260.13783508323491822No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTT3940.12748127416562857No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGGGGTTTTTTGT3940.12748127416562857No Hit
GTTGGTTTTTTTGGGGTGAAGCGGTTTTTATTTTCGTTGATGAATGGTTT3890.12586349149855208No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT3860.12489282189830618No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTTGGAGGAGAGG3820.12359859576464498No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGCGTAGGCGGTGTTGAT3720.12036303043049196No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTCGTTT3630.11745102162975425No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTAGTTTTATTTTTAAATTT3610.11680390856292366No Hit
TTTTTTTTGGGTTTTTAGGGTAGAGTTCGGGTTGGGGAGGTTAGTGTTTC3460.11195056056169414No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTTTGTTATTTTACGATGAGTTAG3220.10418520375972692No Hit
GAATTTTGGGTTTGATTTTTGTTTCGGTTCGGGGTGTAGGTGGCGTTGAT3180.10289097762606571No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTATGGGATAGGGGGTAT3160.10224386455923512No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)