FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309065
Sequences flagged as poor quality0
Sequence length301
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA94113.044990535971398No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA85692.7725559348357143No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT83772.7104330804199765No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT77832.5182404995712875No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG73392.374581398734894No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC72862.357432902463883No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC70932.29498649151473No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA70542.282367786711533No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC64872.098911232265057No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC64762.095352110397489No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC63962.069467587724265No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT61031.974665523433582No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA59841.9361622959571612No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT58821.9031595295488004No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC58021.8772750068755764No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC56471.8271237441962045No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT53461.7297332276381991No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA52761.707084270299128No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA52341.6934948958956852No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC52241.6902593305615325No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA52021.6831410868263958No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG50761.6423729636160678No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA38661.2508695581835536No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC33601.0871499522754113No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC32861.063206768802679No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT31831.029880445860903No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA31781.0282626631938265No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA31601.022438645592351No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA29520.9551388866419686No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA29350.9496384255739084No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA28180.9117823111643182No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA27460.8884862407584164No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC25700.8315402908773235No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT23510.7606814100593726No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA21420.6930580945755748No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT21330.690146085774837No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC20730.670732693769919No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC16280.52675003640011No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT14620.47303965185317004No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA14580.47174542571950884No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATGTCG14510.46948052998560175No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC13420.43421286784333396No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC11380.3682073350266125No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA10860.3513823952890169No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA10480.3390872470192354No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT10290.33293967288434473No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA10080.3261449856826234No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCGCACCAACAT9750.31546762007991846No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCACTCTAACCACCCTCG9360.30284891527672175No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA8690.28117062753789657No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA8310.26887547926811517No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC8140.263375018200055No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC8050.26046300939931727No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA7890.25528610486467246No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT7650.24752074806270527No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA6890.2229304515231424No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATGA6660.21548865125459046No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCA6450.2086939640528691No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCTAACTCATTCCACACCAACAT5550.17957387604549205No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT5350.17310274537718603No Hit
TAAAACTAAACCCCACATACCTTCCCCAACCCAAACACAACACTAACACA5270.1705142931098636No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTGCCTCCGCCT5180.1676022843091259No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA5070.1640431624415576No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC4810.15563069257275977No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACAAAAAAAATAATCACA4770.15433646643909857No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTGCTAAATACACAACC4670.15110090110494556No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC4590.14851244883762316No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCGCC4520.14624755310371604No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAGAACCTTCATC4430.14333554430297832No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA4370.14139420510248654No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA4320.13977642243541002No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4110.13298173523368872No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC4100.1326581787002734No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT3960.1281283872324592No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCACTTACCAATCCACTC3940.12748127416562857No Hit
ATCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC3710.12003947389707667No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC3600.11648035202950835No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA3550.11486256936243185No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC3530.11421545629560126No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC3530.11421545629560126No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC3430.11097989096144824No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT3400.11000922136120235No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACACTACCTT3360.10871499522754112No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA3300.10677365602704933No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG3210.10386164722631162No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)